Cbp Antibody (G-8): sc-365387

Cbp Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Cbp protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Cbp (G-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cbp, also known as Csk-binding protein or phosphoprotein associated with glycosphingolipid-enriched microdomains, plays a crucial role in regulating Src family protein tyrosine kinases (Src-PTKs), which are vital for growth and differentiation of eukaryotic cells. Cbp is primarily localized in GM1 ganglioside-enriched detergent-insoluble membrane domain of plasma membrane, a location essential for effective receptor-mediated signaling. By anchoring Csk to membrane, Cbp facilitates c-Src inhibition, contributing to regulatory mechanisms that control membrane-associated Src-PTKs activity. This interaction helps maintain cellular homeostasis and prevents uncontrolled cell proliferation, making Cbp a key player in various signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cbp Antibody (G-8) References:

1. Cutting edge: Fyn is essential for tyrosine phosphorylation of Csk-binding protein/phosphoprotein associated with glycolipid-enriched microdomains in lipid rafts in resting T cells.  |  Yasuda, K., et al. 2002. J Immunol. 169: 2813-7. PMID: 12218089
2. Transmembrane adaptor proteins in membrane microdomains: important regulators of immunoreceptor signaling.  |  Horejsí, V. 2004. Immunol Lett. 92: 43-9. PMID: 15081526
3. Paxillin family members function as Csk-binding proteins that regulate Lyn activity in human and murine platelets.  |  Rathore, VB., et al. 2007. Biochem J. 403: 275-81. PMID: 17233630
4. Src family kinases: regulation of their activities, levels and identification of new pathways.  |  Ingley, E. 2008. Biochim Biophys Acta. 1784: 56-65. PMID: 17905674
5. The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1.  |  Veracini, L., et al. 2008. J Cell Biol. 182: 603-14. PMID: 18695048
6. Down-regulation of the tumor suppressor C-terminal Src kinase (Csk)-binding protein (Cbp)/PAG1 is mediated by epigenetic histone modifications via the mitogen-activated protein kinase (MAPK)/phosphatidylinositol 3-kinase (PI3K) pathway.  |  Suzuki, K., et al. 2011. J Biol Chem. 286: 15698-706. PMID: 21388951
7. Presenilin1/gamma-secretase promotes the EphB2-induced phosphorylation of ephrinB2 by regulating phosphoprotein associated with glycosphingolipid-enriched microdomains/Csk binding protein.  |  Georgakopoulos, A., et al. 2011. FASEB J. 25: 3594-604. PMID: 21746865
8. Modulation of T cell immune functions by the prostaglandin E(2) - cAMP pathway in chronic inflammatory states.  |  Brudvik, KW. and Taskén, K. 2012. Br J Pharmacol. 166: 411-9. PMID: 22141738
9. Targeting Lyn tyrosine kinase through protein fusions encompassing motifs of Cbp (Csk-binding protein) and the SOCS box of SOCS1.  |  Whiting, RJ., et al. 2012. Biochem J. 442: 611-20. PMID: 22364282
10. SUMOylation of Csk Negatively Modulates its Tumor Suppressor Function.  |  Cui, N., et al. 2019. Neoplasia. 21: 676-688. PMID: 31125786

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cbp Antibody (G-8) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Cbp Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cbp Antibody (G-8) Specifications

Alternate Product Name: PAG1

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 333-432

Epitope Target: Cbp

Epitope Species: human

Product Reactivity/Detection: Cbp

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Cbp Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin