CBARA1/MICU1 Antibody (D-10): sc-518183
- CBARA1/MICU1 Antibody (D-10) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 304-327 of CBARA1 of human origin
- recommended for detection of CBARA1 of human origin by WB, IP, IF and ELISA
- also available conjugated to Alexa Fluor® 488 (sc-518183 AF488, 200 µg/ml) for IF, IHC(P) and FCM
- m-IgGκ BP-HRP is the preferred secondary detection reagent for CBARA1/MICU1 Antibody (D-10) for WB applications. This reagent is now offered in a bundle with CBARA1/MICU1 Antibody (D-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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CBARA1 Antibody (D-10) is a mouse monoclonal IgM antibody that detects CBARA1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CBARA1 (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CBARA1, also known as calcium binding atopy-related autoantigen 1 or CALC, is a 476 amino acid single-pass membrane protein characterized by two EF-hand domains, which are crucial for calcium ion binding. The EF-hand domain′s structure, consisting of a twelve amino acid loop motif that typically forms a stable four-helix bundle, is essential for CBARA1′s ability to interact with calcium ions, thereby influencing various cellular processes. CBARA1 is predominantly expressed in epidermal keratinocytes and dermal endothelial cells, where CBARA1 plays a significant role in inducing T cell-mediated autoreactivity. This autoreactivity is associated with interferon-gamma (IFN-γ) release and can trigger allergic reactions, leading to immunoglobulin E (IgE) formation. IgE binding to otherwise harmless environmental antigens can result in cross-linking with IgE receptors, a mechanism linked to atopic dermatitis pathogenesis. Multiple isoforms of CBARA1 arise from alternative splicing, further diversifying CBARA1′s functional roles in immune responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CBARA1/MICU1 Antibody (D-10) References:
- Hom s 4, an IgE-reactive autoantigen belonging to a new subfamily of calcium-binding proteins, can induce Th cell type 1-mediated autoreactivity. | Aichberger, KJ., et al. 2005. J Immunol. 175: 1286-94. PMID: 16002733
- Evolution of EF-hand calcium-modulated proteins. II. Domains of several subfamilies have diverse evolutionary histories. | Nakayama, S., et al. 1992. J Mol Evol. 34: 416-48. PMID: 1602495
- Evolution of EF-hand calcium-modulated proteins. I. Relationships based on amino acid sequences. | Moncrief, ND., et al. 1990. J Mol Evol. 30: 522-62. PMID: 2115931
- Calcium-binding proteins 1: EF-hands. | Kawasaki, H. and Kretsinger, RH. 1995. Protein Profile. 2: 297-490. PMID: 7553064
- Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. | Maruyama, K. and Sugano, S. 1994. Gene. 138: 171-4. PMID: 8125298
- Isolation of cDNA clones coding for IgE autoantigens with serum IgE from atopic dermatitis patients. | Natter, S., et al. 1998. FASEB J. 12: 1559-69. PMID: 9806765
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CBARA1/MICU1 Antibody (D-10) | sc-518183 | 200 µg/ml | $322.00 | |||
CBARA1/MICU1 Antibody (D-10): m-IgGκ BP-HRP Bundle | sc-550861 | 200 µg Ab; 40 µg BP | $361.00 | |||
CBARA1/MICU1 Antibody (D-10) Alexa Fluor® 488 | sc-518183 AF488 | 200 µg/ml | $364.00 |