Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
cathepsin Z CRISPR/Cas9 KO Plasmid (h): sc-403244
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cathepsin Z CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the cathepsin Z genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cathepsin Z Antibody (F-6): sc-376976
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cathepsin Z CRISPR/Cas9 KO Plasmid (h) | sc-403244 | 20 µg | $397.00 |
Overview
CTSZ encodes cathepsin Z (cathepsin X), a lysosomal cysteine protease that participates in intracellular protein turnover and peptide processing within the endo-lysosomal system. Cathepsin Z can modulate cell adhesion and migration through interactions involving its RGD motif and by shaping protease networks that remodel the extracellular milieu. Its activity is linked to antigen processing and inflammatory signaling in immune cells, connecting CTSZ to pathways governing innate and adaptive immune responses. Dysregulated CTSZ expression or activity has been associated with tumor invasion phenotypes and neuroinflammatory or neurodegenerative contexts, making it a useful target for mechanistic studies of proteostasis and immune microenvironments.
cathepsin Z CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CTSZ gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CTSZ together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CTSZ open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish cathepsin Z protein expression.
This CRISPR knockout system enables efficient generation of CTSZ-deficient cell models for investigation of cathepsin Z signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CTSZ exon(s) critical for cathepsin Z function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CTSZ genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by cathepsin Z CRISPR/Cas9 KO Plasmid (h) and cathepsin Z CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CTSZ locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by cathepsin Z HDR Plasmid (h) and cathepsin Z HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CTSZ homology arms to support homology-directed repair at defined CTSZ target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.