cathepsin W Antibody (BV39-2B): sc-58337

Cathepsin W Antibody (BV39-2B) is a mouse monoclonal IgG2b antibody that detects cathepsin W in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Cathepsin W, also known as lymphopain, is a member of a unique subgroup of cathepsin proteases that are phylogenetically distinct from other human cathepsins, and plays a crucial role in the immune response by being specifically expressed in CD8+ T lymphocytes. The expression of cathepsin W is initiated during the differentiation of thyrocytes into CD8+ T lymphocytes, coinciding with the downregulation of CD4+ receptors in thymocytes, which is vital for proper development of T cell subsets. Cathepsin W is primarily localized within the rough endoplasmic reticulum in transfected Cos-7 and HeLa cells, highlighting its importance in protein processing and secretion. Structurally, cathepsin W features a unique 21 amino acid peptide insertion between the active site histidine and asparagine residues, along with a distinctive 8-amino acid carboxy-terminal extension. Additionally, cathepsin W possesses an extended loop structure in the beta-sheet domain and an extra disulfide bond, which contribute to its stability and function. Cathepsin W may also exist as a dimer, with each monomer linked by a disulfide bond, further emphasizing its complex structural characteristics that are essential for enzymatic activity and interactions within the immune system.

The preferred cathepsin W antibody is cathepsin W Antibody (1B1): sc-32799.