cathepsin E Antibody (D-8): sc-166500
- cathepsin E Antibody (D-8) is a mouse monoclonal IgG1 κ cathepsin E antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 376-395 at the C-terminus of cathepsin E of human origin
- cathepsin E Antibody (D-8) is recommended for detection of cathepsin E of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-cathepsin E Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for cathepsin E Antibody (D-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with cathepsin E Antibody (D-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Cathepsin E Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects cathepsin E protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-cathepsin E antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cathepsin E, a member of the aspartyl protease class, plays a crucial role in the immune system by processing antigens and is involved in the maturation of certain immune cells. Non-lysosomal localization allows cathepsin E to participate in intracellular protein degradation and peptide biosynthesis outside the lysosome, which is essential for maintaining cellular homeostasis and regulating immune responses. Interactions between cathepsin E and various proteins, including those involved in antigen presentation, underscore cathepsin E′s importance in immune function and potential implications in autoimmune diseases and cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cathepsin E Antibody (D-8) References:
- Molecular cloning and expression of human alveolar macrophage cathepsin S, an elastinolytic cysteine protease. | Shi, GP., et al. 1992. J Biol Chem. 267: 7258-62. PMID: 1373132
- Cathepsin D, but not cathepsin E, degrades desmosomes during epidermal desquamation. | Igarashi, S., et al. 2004. Br J Dermatol. 151: 355-61. PMID: 15327542
- An immunocytochemical study on distinct intracellular localization of cathepsin E and cathepsin D in human gastric cells and various rat cells. | Saku, T., et al. 1991. J Biochem. 110: 956-64. PMID: 1794985
- Cathepsin E enhances anticancer activity of doxorubicin on human prostate cancer cells showing resistance to TRAIL-mediated apoptosis. | Yasukochi, A., et al. 2010. Biol Chem. 391: 947-58. PMID: 20482316
- Molecular organization of the human cathepsin D gene. | Redecker, B., et al. 1991. DNA Cell Biol. 10: 423-31. PMID: 2069717
- Molecular cloning and sequencing of cDNA for rat cathepsin L. | Ishidoh, K., et al. 1987. FEBS Lett. 223: 69-73. PMID: 3666143
- Molecular cloning and sequencing of cDNA for rat cathepsin H. Homology in pro-peptide regions of cysteine proteinases. | Ishidoh, K., et al. 1987. FEBS Lett. 226: 33-7. PMID: 3691815
- Isolation and sequencing of two cDNA clones encoding rat spleen cathepsin E and analysis of the activation of purified procathepsin E. | Okamoto, K., et al. 1995. Arch Biochem Biophys. 322: 103-11. PMID: 7574663
- Molecular cloning of human cathepsin O, a novel endoproteinase and homologue of rabbit OC2. | Shi, GP., et al. 1995. FEBS Lett. 357: 129-34. PMID: 7805878
- Cathepsin B, a cysteine protease implicated in metastatic progression, is also expressed during regression of the rat prostate and mammary glands. | Guenette, RS., et al. 1994. Eur J Biochem. 226: 311-21. PMID: 8001549
- Molecular cloning, chromosomal location, and tissue-specific expression of the murine cathepsin G gene. | Heusel, JW., et al. 1993. Blood. 81: 1614-23. PMID: 8453108
- Mouse cathepsin K: cDNA cloning and predominant expression of the gene in osteoclasts, and in some hypertrophying chondrocytes during mouse development. | Rantakokko, J., et al. 1996. FEBS Lett. 393: 307-13. PMID: 8814310
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cathepsin E Antibody (D-8) | sc-166500 | 200 µg/ml | $322.00 | |||
cathepsin E Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-521632 | 200 µg Ab, 40 µg BP | $361.00 | |||
cathepsin E Antibody (D-8) AC | sc-166500 AC | 500 µg/ml, 25% agarose | $424.00 | |||
cathepsin E Antibody (D-8) HRP | sc-166500 HRP | 200 µg/ml | $322.00 | |||
cathepsin E Antibody (D-8) FITC | sc-166500 FITC | 200 µg/ml | $336.00 | |||
cathepsin E Antibody (D-8) PE | sc-166500 PE | 200 µg/ml | $349.00 | |||
cathepsin E Antibody (D-8) Alexa Fluor® 488 | sc-166500 AF488 | 200 µg/ml | $364.00 | |||
cathepsin E Antibody (D-8) Alexa Fluor® 546 | sc-166500 AF546 | 200 µg/ml | $364.00 | |||
cathepsin E Antibody (D-8) Alexa Fluor® 594 | sc-166500 AF594 | 200 µg/ml | $364.00 | |||
cathepsin E Antibody (D-8) Alexa Fluor® 647 | sc-166500 AF647 | 200 µg/ml | $364.00 | |||
cathepsin E Antibody (D-8) Alexa Fluor® 680 | sc-166500 AF680 | 200 µg/ml | $364.00 | |||
cathepsin E Antibody (D-8) Alexa Fluor® 790 | sc-166500 AF790 | 200 µg/ml | $364.00 | |||
cathepsin E (D-8) Neutralizing Peptide | sc-166500 P | 100 µg/0.5 ml | $69.00 |