Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
caspase-7 CRISPR/Cas9 KO Plasmid (m): sc-419468
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- caspase-7 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the caspase-7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: caspase-7 p10 Antibody (B-3): sc-365034
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caspase-7 CRISPR/Cas9 KO Plasmid (m) | sc-419468 | 20 µg | $397.00 |
Overview
Mouse Casp7 encodes caspase-7, an executioner cysteine-aspartate protease that is activated downstream of initiator caspases during intrinsic and extrinsic apoptosis. Once processed, caspase-7 cleaves multiple cellular substrates to drive characteristic apoptotic morphology and contributes to regulated cell death programs linked to mitochondrial signaling, death receptor pathways, and proteostasis stress. Caspase-7 activity interfaces with inflammatory and immune signaling through crosstalk with caspase networks and can influence tissue remodeling and responses to cytotoxic stress. Dysregulated apoptosis involving CASP7 has been implicated in phenotypes relevant to cancer biology, neurodegeneration, and immune-mediated pathology, making Casp7 a useful node for mechanistic studies of cell fate control.
caspase-7 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Casp7 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Casp7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Casp7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish caspase-7 protein expression.
This CRISPR knockout system enables efficient generation of Casp7-deficient cell models for investigation of caspase-7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Casp7 exon(s) critical for caspase-7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Casp7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by caspase-7 CRISPR/Cas9 KO Plasmid (m) and caspase-7 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Casp7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by caspase-7 HDR Plasmid (m) and caspase-7 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Casp7 homology arms to support homology-directed repair at defined Casp7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.