caspase-14 CRISPR/Cas9 KO Plasmid (m): sc-419464
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- caspase-14 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the caspase-14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: caspase-14 p10 Antibody (B-3): sc-515259
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caspase-14 CRISPR/Cas9 KO Plasmid (m) | sc-419464 | 20 µg | $397.00 |
Overview
Mouse Casp14 encodes caspase-14, an atypical member of the caspase family that contributes to terminal keratinocyte differentiation rather than apoptotic signaling. Caspase-14 activity supports epidermal barrier formation by promoting processing of differentiation-associated substrates and influencing filaggrin-derived natural moisturizing factors in the stratum corneum. Through its role in cornification and skin homeostasis, altered Casp14 expression or activity has been associated with impaired barrier function and inflammatory skin phenotypes in experimental models. Caspase-14 therefore provides a mechanistic entry point for studying epithelial differentiation programs, barrier stress responses, and keratinization-linked pathways.
caspase-14 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Casp14 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Casp14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Casp14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish caspase-14 protein expression.
This CRISPR knockout system enables efficient generation of Casp14-deficient cell models for investigation of caspase-14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Casp14 exon(s) critical for caspase-14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Casp14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by caspase-14 CRISPR/Cas9 KO Plasmid (m) and caspase-14 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Casp14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by caspase-14 HDR Plasmid (m) and caspase-14 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Casp14 homology arms to support homology-directed repair at defined Casp14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.