Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
caspase-12 CRISPR/Cas9 KO Plasmid (m): sc-419463
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- caspase-12 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the caspase-12 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: caspase-12 Antibody (1611): sc-21747
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caspase-12 CRISPR/Cas9 KO Plasmid (m) | sc-419463 | 20 µg | $397.00 |
Overview
Mouse Casp12 encodes caspase-12, an inflammatory caspase implicated in innate immune signaling and modulation of protease cascades linked to cellular stress and inflammatory responses. Caspase-12 has been associated with endoplasmic reticulum stress pathways and can influence signaling nodes that shape cytokine production and antimicrobial defenses. In murine models, Casp12 activity has been studied in the context of host–pathogen interactions and inflammation-related tissue damage. Its functional relationship to other caspases and stress-response pathways makes it relevant for investigating mechanisms underlying inflammatory phenotypes in disease models.
caspase-12 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Casp12 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Casp12 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Casp12 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish caspase-12 protein expression.
This CRISPR knockout system enables efficient generation of Casp12-deficient cell models for investigation of caspase-12 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Casp12 exon(s) critical for caspase-12 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Casp12 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by caspase-12 CRISPR/Cas9 KO Plasmid (m) and caspase-12 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Casp12 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by caspase-12 HDR Plasmid (m) and caspase-12 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Casp12 homology arms to support homology-directed repair at defined Casp12 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.