caspase-1 p20 Antibody (D-4): sc-398715

caspase-1 p20 Antibody (D-4) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect the p20 subunit and precursor forms of caspase-1, also known as IL-1 converting enzyme (ICE), in mouse and rat samples. anti-caspase-1 p20 antibody (D-4) targets an epitope spanning amino acids 259-294 at the C-terminus of the caspase-1 p20 subunit, ensuring precise recognition in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Caspase-1 is a critical protease involved in the inflammatory response and apoptosis, where its structure plays a pivotal role in its function. The enzyme comprises a prodomain (Pro-C1) and two catalytic subunits, p20 and p10, which assemble into an active (p20/p10)_2 tetramer. This structural configuration is essential for the proteolytic cleavage of pro-inflammatory cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18), as well as for initiating apoptotic pathways by dismantling cellular components. Understanding the structural integrity of caspase-1 is vital for elucidating its role in various physiological and pathological processes, including inflammation, septic shock, wound healing, and certain leukemias. The human CASP1 gene, located on chromosome 2q14, encodes a cytoplasmic protein expressed in tissues such as the liver, heart, skeletal muscle, kidney, and testis. By providing reliable detection of both the precursor and active p20 forms of caspase-1, caspase-1 p20 (D-4) monoclonal antibody facilitates comprehensive studies into the enzyme′s structural dynamics and its implications in disease mechanisms. Available in both non-conjugated and various conjugated forms, caspase-1 p20 (D-4) antibody is an indispensable tool for researchers investigating the multifaceted roles of caspase-1 in cellular regulation and immune responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

caspase-1 p20 Antibody (D-4) References:

1. Cloning and sequencing of feline and canine ice-related cDNAs encoding hybrid caspase-1/caspase-13-like propeptides.  |  Taylor, S., et al. 2000. DNA Seq. 10: 387-94. PMID: 10826695
2. The most unkindest cut of all: on the multiple roles of mammalian caspases.  |  Fadeel, B., et al. 2000. Leukemia. 14: 1514-25. PMID: 10942252
3. Noncaspase proteases in apoptosis.  |  Johnson, DE. 2000. Leukemia. 14: 1695-703. PMID: 10995018
4. Caspase pathways, neuronal apoptosis, and CNS injury.  |  Eldadah, BA. and Faden, AI. 2000. J Neurotrauma. 17: 811-29. PMID: 11063050
5. Proteases for cell suicide: functions and regulation of caspases.  |  Chang, HY. and Yang, X. 2000. Microbiol Mol Biol Rev. 64: 821-46. PMID: 11104820
6. Caspases: key players in programmed cell death.  |  Grütter, MG. 2000. Curr Opin Struct Biol. 10: 649-55. PMID: 11114501
7. Signaling for the caspases: their role in prostate cell apoptosis.  |  Coffey, RN., et al. 2001. J Urol. 165: 5-14. PMID: 11125352
8. Overexpression of Caspase-1 in adenocarcinoma of pancreas and chronic pancreatitis.  |  Yang, YM., et al. 2003. World J Gastroenterol. 9: 2828-31. PMID: 14669344
9. Expression, refolding, and autocatalytic proteolytic processing of the interleukin-1 beta-converting enzyme precursor.  |  Ramage, P., et al. 1995. J Biol Chem. 270: 9378-83. PMID: 7721861

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-1 p20 Antibody (D-4) has 119 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-1 p20 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-1 p20 Antibody (D-4) Specifications

Alternate Product Name: caspase-1 p20

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 259-294

Epitope Target: caspase-1 p20

Epitope Species: mouse

Product Reactivity/Detection: caspase-1 precursor and p20 subunit

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 119 citations in various scientific publications

Available Conjugates/Forms for caspase-1 p20 Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred caspase-1 antibody is caspase-1 Antibody (14F468): sc-56036.