CAS4 Antibody (B-1): sc-514771

CAS4 Antibody (B-1) is a mouse monoclonal IgG1 antibody that detects CAS4 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CAS4, also known as CASS4, HEFL, HEPL, or C20orf32, is a 786 amino acid protein that plays a crucial role in cellular signaling pathways, particularly those related to cell adhesion. CAS4 is primarily localized in the cytoplasm and cytoskeleton, with significant expression levels found in the lung and spleen, as well as in ovarian and leukemia cell lines. CAS4′s localization enables interaction with focal adhesion kinase (FAK) via its C-terminal SH3 domain, facilitating tyrosine-kinase-based signaling essential for maintaining proper cell adhesion and communication. Additionally, CAS4 undergoes post-translational modifications, including phosphorylation on tyrosines by SRC, which further enhances functional capacity as a docking protein in signaling cascades. Multiple isoforms of CAS4 arise from alternative splicing events, adding complexity to regulatory mechanisms. The gene encoding CAS4 is located on human chromosome 20, which comprises about 2% of the human genome and contains approximately 600 genes, underscoring the significance in various biological processes. Anti-CAS4 antibody (B-1) serves as an invaluable tool for researchers investigating the intricate roles of CAS4 in cellular dynamics and disease states.

The preferred CAS4 antibody is CAS4 Antibody (H-3): sc-376968.