CAS Antibody (H-2): sc-271537

CAS Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects CAS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CAS (H-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Cellular Apoptosis Susceptibility protein (CAS), also known as Exportin 2, is a crucial 971 amino acid member of the CSE1 family that plays a vital role in the nuclear transport of proteins. Specifically, CAS mediates the re-export of Importin α from the nucleus to the cytoplasm after releasing its cargo into the nucleoplasm. This process is essential for maintaining cellular homeostasis and regulating apoptosis, as CAS ensures proteins are properly shuttled in and out of the nucleus, thereby influencing gene expression and cell survival. In the nucleus, CAS forms a complex with Importin α and the GTPase Ran in its active GTP-bound form, facilitating the docking of this complex to the nuclear pore complex through interactions with nucleoporins. Once in the cytoplasm, the complex dissociates, allowing Importin α to be released while CAS returns to the nucleus to repeat the cycle. Notably, CAS shows high expression in proliferating cells, and three isoforms of CAS have been identified due to alternative splicing, with Isoform 1 being the full-length protein, while Isoforms 2 and 3 exhibit variations in their amino acid sequences and lengths.

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CAS Antibody (H-2) References:

1. RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex.  |  Hale, CR., et al. 2009. Cell. 139: 945-56. PMID: 19945378
2. Presence of secretory cellular apoptosis susceptibility protein in cerebrospinal fluids of patients with intracerebral hemorrhage caused by stroke and neurotrauma.  |  Tung, JN., et al. 2010. Neuro Endocrinol Lett. 31: 390-8. PMID: 20588233
3. NSP-Cas protein structures reveal a promiscuous interaction module in cell signaling.  |  Mace, PD., et al. 2011. Nat Struct Mol Biol. 18: 1381-7. PMID: 22081014
4. Cloning and characterization of a cellular apoptosis susceptibility gene, the human homologue to the yeast chromosome segregation gene CSE1.  |  Brinkmann, U., et al. 1995. Proc Natl Acad Sci U S A. 92: 10427-31. PMID: 7479798
5. Survival factors, intracellular signal transduction, and the activation of endonucleases in apoptosis.  |  Eastman, A. 1995. Semin Cancer Biol. 6: 45-52. PMID: 7548841
6. Implications of apoptotic cell death regulation in cancer therapy.  |  McDonnell, TJ., et al. 1995. Semin Cancer Biol. 6: 53-60. PMID: 7548842
7. The biochemistry of programmed cell death.  |  Kroemer, G., et al. 1995. FASEB J. 9: 1277-87. PMID: 7557017
8. Out-of body experiences: cell-free cell death.  |  Hengartner, MO. 1995. Bioessays. 17: 549-52. PMID: 7575497
9. Cell cycle and apoptosis: common pathways to life and death.  |  King, KL. and Cidlowski, JA. 1995. J Cell Biochem. 58: 175-80. PMID: 7673325
10. Cell death: current difficulties in discriminating apoptosis from necrosis in the context of pathological processes in vivo.  |  Columbano, A. 1995. J Cell Biochem. 58: 181-90. PMID: 7673326
11. Localization of the cell proliferation and apoptosis-associated CAS protein in lymphoid neoplasms.  |  Wellmann, A., et al. 1997. Am J Pathol. 150: 25-30. PMID: 9006318
12. The hCSE1/CAS protein is phosphorylated by HeLa extracts and MEK-1: MEK-1 phosphorylation may modulate the intracellular localization of CAS.  |  Scherf, U., et al. 1998. Biochem Biophys Res Commun. 250: 623-8. PMID: 9784396

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CAS Antibody (H-2) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-CAS Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CAS Antibody (H-2) Specifications

Alternate Product Name: CAS

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 672-971

Epitope Target: CAS

Epitope Species: human

Product Reactivity/Detection: CAS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for CAS Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin