CARMIL Antibody (E-10): sc-365314
- CARMIL Antibody (E-10) is a mouse monoclonal IgG1 κ CARMIL antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 662-961 mapping within an internal region of CARMIL of human origin
- CARMIL Antibody (E-10) is recommended for detection of CARMIL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CARMIL Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CARMIL Antibody (E-10) for WB and IHC(P) applications. These reagents are now offered in bundles with CARMIL Antibody (E-10) (see ordering information below).
QUICK LINKS
SEE ALSO...
CARMIL Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects CARMIL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CARMIL Antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CARMIL protein, also known as leucine-rich repeat containing 16 (LRRC16), plays a critical role in cellular dynamics by interacting with key proteins such as the Arp2/3 complex, actin capping protein, and Myosin I. These interactions are essential for the assembly of a multi-protein complex that regulates actin filament dynamics, which is vital for processes like cytokinesis, phagocytosis, and muscle contraction. Proper functioning of CARMIL is crucial, as defects in CARMIL gene can lead to significant cellular dysfunctions, including impaired chemotactic aggregation and reduced rates of pinocytosis. Furthermore, without adequate CARMIL activity, assembly of the Myosin-Arp2/3-capping protein complex is compromised, ultimately hindering effective actin filament assembly and disrupting normal cell development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CARMIL Antibody (E-10) References:
- The Dictyostelium CARMIL protein links capping protein and the Arp2/3 complex to type I myosins through their SH3 domains. | Jung, G., et al. 2001. J Cell Biol. 153: 1479-97. PMID: 11425877
- CARMIL is a bona fide capping protein interactant. | Remmert, K., et al. 2004. J Biol Chem. 279: 3068-77. PMID: 14594951
- Mammalian CARMIL inhibits actin filament capping by capping protein. | Yang, C., et al. 2005. Dev Cell. 9: 209-21. PMID: 16054028
- Presence of a novel inhibitor of capping protein in neutrophil extract. | Huang, M., et al. 2005. Cell Motil Cytoskeleton. 62: 232-43. PMID: 16276529
- CARMIL is a potent capping protein antagonist: identification of a conserved CARMIL domain that inhibits the activity of capping protein and uncaps capped actin filaments. | Uruno, T., et al. 2006. J Biol Chem. 281: 10635-50. PMID: 16434392
- Identification of a novel inhibitory actin-capping protein binding motif in CD2-associated protein. | Bruck, S., et al. 2006. J Biol Chem. 281: 19196-203. PMID: 16707503
- The role of CKIP-1 in cell morphology depends on its interaction with actin-capping protein. | Canton, DA., et al. 2006. J Biol Chem. 281: 36347-59. PMID: 16987810
- The extracellular leucine-rich repeat superfamily; a comparative survey and analysis of evolutionary relationships and expression patterns. | Dolan, J., et al. 2007. BMC Genomics. 8: 320. PMID: 17868438
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CARMIL Antibody (E-10) | sc-365314 | 200 µg/ml | $322.00 | |||
CARMIL Antibody (E-10): m-IgG Fc BP-HRP Bundle | sc-527353 | 200 µg Ab; 10 µg BP | $361.00 | |||
CARMIL Antibody (E-10): m-IgG1 BP-HRP Bundle | sc-532726 | 200 µg Ab; 20 µg BP | $361.00 | |||
CARMIL Antibody (E-10) AC | sc-365314 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CARMIL Antibody (E-10) HRP | sc-365314 HRP | 200 µg/ml | $322.00 | |||
CARMIL Antibody (E-10) FITC | sc-365314 FITC | 200 µg/ml | $336.00 | |||
CARMIL Antibody (E-10) PE | sc-365314 PE | 200 µg/ml | $349.00 | |||
CARMIL Antibody (E-10) Alexa Fluor® 488 | sc-365314 AF488 | 200 µg/ml | $364.00 | |||
CARMIL Antibody (E-10) Alexa Fluor® 546 | sc-365314 AF546 | 200 µg/ml | $364.00 | |||
CARMIL Antibody (E-10) Alexa Fluor® 594 | sc-365314 AF594 | 200 µg/ml | $364.00 | |||
CARMIL Antibody (E-10) Alexa Fluor® 647 | sc-365314 AF647 | 200 µg/ml | $364.00 | |||
CARMIL Antibody (E-10) Alexa Fluor® 680 | sc-365314 AF680 | 200 µg/ml | $364.00 | |||
CARMIL Antibody (E-10) Alexa Fluor® 790 | sc-365314 AF790 | 200 µg/ml | $364.00 |