Cardiotin Antibody (SR-3): sc-58711

Cardiotin Antibody (SR-3) is a mouse monoclonal IgM antibody that detects Cardiotin in human and porcine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Cardiotin is a high molecular weight protein complex located in the mitochondrial membrane, where Cardiotin plays a crucial role in maintaining mitochondrial function and energy production, essential for proper functioning of cardiac cells. Cardiotin′s structure consists of two subunits that share a highly homologous N-terminal 14 amino acid sequence with human skeletal muscle α-actinin, indicating that Cardiotin forms a tetrameric transmembrane complex with its N-terminus positioned on the cytoplasmic side of the membrane, allowing Cardiotin to interact effectively with actin filaments. This interaction is vital for the structural integrity and contractile function of cardiomyocytes. Notably, during cardiac contractile dysfunction, Cardiotin distribution is altered in pathological cardiomyocytes, such as those found in chronic ischemic myocardium, making anti-Cardiotin antibody (SR-3) an invaluable tool for researchers investigating disturbed mitochondrial activity in conditions like chronic ischemia or chronic atrial fibrillation.

The preferred Cardiotin antibody is Cardiotin Antibody (R2G): sc-53001.