CAR Antibody (E-1): sc-373791

CAR Antibody (E-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects CAR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CAR antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The coxsackie and adenovirus receptor (CAR) plays a crucial role in mediating viral infections by facilitating the binding of various adenoviruses through specific protein interactions. CAR is predominantly located on the surface of epithelial cells, where expression levels directly influence adenoviral infection efficiency. Cells expressing low CAR levels show reduced susceptibility to adenoviral entry, creating challenges for gene therapy applications using adenoviral vectors. Understanding CAR cellular localization remains essential for optimizing viral delivery systems and enhancing gene therapy strategies. Targeting CAR expression or modifying adenoviral knobs to interact with alternative receptors can potentially overcome limitations posed by low CAR expression in certain cell types, improving therapeutic outcomes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CAR Antibody (E-1) References:

1. CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts.  |  Hidaka, C., et al. 1999. J Clin Invest. 103: 579-87. PMID: 10021467
2. Identification of a conserved receptor-binding site on the fiber proteins of CAR-recognizing adenoviridae.  |  Roelvink, PW., et al. 1999. Science. 286: 1568-71. PMID: 10567265
3. Structural analysis of the mechanism of adenovirus binding to its human cellular receptor, CAR.  |  Bewley, MC., et al. 1999. Science. 286: 1579-83. PMID: 10567268
4. Identification of contact residues and definition of the CAR-binding site of adenovirus type 5 fiber protein.  |  Kirby, I., et al. 2000. J Virol. 74: 2804-13. PMID: 10684297
5. Coxsackie-and-adenovirus receptor mRNA expression in human heart failure.  |  Sasse, A., et al. 2003. J Gene Med. 5: 876-882. PMID: 14533196
6. Epigenetic regulation of coxsackie and adenovirus receptor (CAR) gene promoter in urogenital cancer cells.  |  Pong, RC., et al. 2003. Cancer Res. 63: 8680-6. PMID: 14695181
7. Coxsackie and adenovirus receptor binding ablation reduces adenovirus liver tropism and toxicity.  |  Yun, CO., et al. 2005. Hum Gene Ther. 16: 248-61. PMID: 15761264
8. Impact of the coxsackievirus and adenovirus receptor on the adenoma-carcinoma sequence of colon cancer.  |  Stecker, K., et al. 2011. Br J Cancer. 104: 1426-33. PMID: 21468049
9. Naturally occurring variants in the transmembrane and cytoplasmic domains of the human Coxsackie- and adenovirus receptor have no impact on virus internalisation.  |  Herrmann, L., et al. 2020. Biochem Biophys Res Commun. 527: 401-405. PMID: 32334832
10. Extracellular Vesicles-Mimetic Encapsulation Improves Oncolytic Viro-Immunotherapy in Tumors With Low Coxsackie and Adenovirus Receptor.  |  Zhang, Y., et al. 2020. Front Bioeng Biotechnol. 8: 574007. PMID: 33042975
11. An adenovirus vector with genetically modified fibers demonstrates expanded tropism via utilization of a coxsackievirus and adenovirus receptor-independent cell entry mechanism.  |  Dmitriev, I., et al. 1998. J Virol. 72: 9706-13. PMID: 9811704
12. Coxsackievirus and adenovirus receptor amino-terminal immunoglobulin V-related domain binds adenovirus type 2 and fiber knob from adenovirus type 12.  |  Freimuth, P., et al. 1999. J Virol. 73: 1392-8. PMID: 9882344

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CAR Antibody (E-1) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-CAR Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CAR Antibody (E-1) Specifications

Alternate Product Name: Coxsackie virus Adenovirus Receptor

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: CAR

Epitope Species: human

Product Reactivity/Detection: CAR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for CAR Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin