CapZ-α Antibody (H-9): sc-374302
- CapZ-α Antibody (H-9) is a mouse monoclonal IgG1 κ CapZ-α antibody provided at 200 µg/ml
- raised against amino acids 157-286 mapping at the C-terminus of CapZ-α1 of human origin
- CapZ-alpha Antibody (H-9) is recommended for detection of CapZ-α1 and CapZ-α2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CapZ-alpha Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CapZ-α (H-9): sc-374302.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CapZ-α Antibody (H-9) for WB and IHC(P) applications. These reagents are now offered in bundles with CapZ-α Antibody (H-9) (see ordering information below).
CapZ-α Antibody (H-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects CapZ-alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-CapZ-alpha antibody (H-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CapZ-alpha protein plays a crucial role in regulating actin dynamics by capping barbed ends of actin filaments, preventing further polymerization and maintaining cellular structure and motility. Such regulation is vital for numerous cellular processes, including muscle contraction, cell migration, and intracellular transport. CapZ-alpha protein forms a heterodimeric complex with a beta subunit, maintaining the balance of actin filament assembly and disassembly critical for cellular integrity and function. CapZ-α1, the alpha subunit of this complex, interacts with various signaling molecules, including S-100 β chain, involved in calcium-sensitive signaling pathways linked to neurodegenerative diseases such as Alzheimer′s. CapZ-α monoclonal antibody (H-9) effectively detects and characterizes this important protein, making this antibody valuable for researchers studying actin dynamics and related cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CapZ-α Antibody (H-9) References:
- Alpha actinin-CapZ, an anchoring complex for thin filaments in Z-line. | Papa, I., et al. 1999. J Muscle Res Cell Motil. 20: 187-97. PMID: 10412090
- Solution NMR structure of S100B bound to the high-affinity target peptide TRTK-12. | Inman, KG., et al. 2002. J Mol Biol. 324: 1003-14. PMID: 12470955
- Linking the T cell surface protein CD2 to the actin-capping protein CAPZ via CMS and CIN85. | Hutchings, NJ., et al. 2003. J Biol Chem. 278: 22396-403. PMID: 12690097
- Arabidopsis capping protein (AtCP) is a heterodimer that regulates assembly at the barbed ends of actin filaments. | Huang, S., et al. 2003. J Biol Chem. 278: 44832-42. PMID: 12947123
- How capping protein binds the barbed end of the actin filament. | Wear, MA., et al. 2003. Curr Biol. 13: 1531-7. PMID: 12956956
- Searching for the missing link: a role for the actin capping protein in heart failure. | Pyle, WG. 2004. Can J Cardiol. 20: 1429-32. PMID: 15614336
- WNT2B: comparative integromics and clinical applications (Review). | Katoh, M. 2005. Int J Mol Med. 16: 1103-8. PMID: 16273293
- New insights into mechanism and regulation of actin capping protein. | Cooper, JA. and Sept, D. 2008. Int Rev Cell Mol Biol. 267: 183-206. PMID: 18544499
- Characterization of S-100b binding epitopes. Identification of a novel target, the actin capping protein, CapZ. | Ivanenkov, VV., et al. 1995. J Biol Chem. 270: 14651-8. PMID: 7540176
- Interaction of Cap Z with actin. The NH2-terminal domains of the alpha 1 and beta subunits are not required for actin capping, and alpha 1 beta and alpha 2 beta heterodimers bind differentially to actin. | Casella, JF. and Torres, MA. 1994. J Biol Chem. 269: 6992-8. PMID: 8120062
- Interaction of S100a0 protein with the actin capping protein, CapZ: characterization of a putative S100a0 binding site in CapZ alpha-subunit. | Ivanenkov, VV., et al. 1996. Biochem Biophys Res Commun. 221: 46-50. PMID: 8660341
- Vertebrates have conserved capping protein alpha isoforms with specific expression patterns. | Hart, MC., et al. 1997. Cell Motil Cytoskeleton. 38: 120-32. PMID: 9331217
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CapZ-α Antibody (H-9) | sc-374302 | 200 µg/ml | $316.00 | |||
CapZ-α Antibody (H-9): m-IgG Fc BP-HRP Bundle | sc-529716 | 200 µg Ab; 10 µg BP | $354.00 | |||
CapZ-α Antibody (H-9): m-IgGκ BP-HRP Bundle | sc-522692 | 200 µg Ab, 40 µg BP | $354.00 | |||
CapZ-α Antibody (H-9): m-IgG1 BP-HRP Bundle | sc-543648 | 200 µg Ab; 20 µg BP | $354.00 | |||
CapZ-α Antibody (H-9) AC | sc-374302 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CapZ-α Antibody (H-9) HRP | sc-374302 HRP | 200 µg/ml | $316.00 | |||
CapZ-α Antibody (H-9) FITC | sc-374302 FITC | 200 µg/ml | $330.00 | |||
CapZ-α Antibody (H-9) PE | sc-374302 PE | 200 µg/ml | $343.00 | |||
CapZ-α Antibody (H-9) Alexa Fluor® 488 | sc-374302 AF488 | 200 µg/ml | $357.00 | |||
CapZ-α Antibody (H-9) Alexa Fluor® 546 | sc-374302 AF546 | 200 µg/ml | $357.00 | |||
CapZ-α Antibody (H-9) Alexa Fluor® 594 | sc-374302 AF594 | 200 µg/ml | $357.00 | |||
CapZ-α Antibody (H-9) Alexa Fluor® 647 | sc-374302 AF647 | 200 µg/ml | $357.00 | |||
CapZ-α Antibody (H-9) Alexa Fluor® 680 | sc-374302 AF680 | 200 µg/ml | $357.00 | |||
CapZ-α Antibody (H-9) Alexa Fluor® 790 | sc-374302 AF790 | 200 µg/ml | $357.00 |