CaMKV CRISPR/Cas9 KO Plasmid (m): sc-433479
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CaMKV CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CaMKV genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CaMKV Antibody (2F3-1A2): sc-517082
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CaMKV CRISPR/Cas9 KO Plasmid (m) | sc-433479 | 20 µg | $397.00 |
Overview
Camkv encodes CaMKV, an atypical calmodulin-dependent protein kinase–like protein enriched in neurons and implicated in activity-dependent signaling. Although reported to have limited or atypical catalytic activity compared with canonical CaMKs, CaMKV participates in Ca2+/calmodulin-linked regulatory networks that influence synaptic signaling, cytoskeletal organization, and neuronal differentiation. Through these processes, Camkv has been connected to pathways governing neurite outgrowth and plasticity, making it relevant to mechanistic studies of nervous system development and dysfunction. Altered regulation of Ca2+-responsive signaling nodes like CaMKV is frequently explored in the context of neurodevelopmental and neuropsychiatric phenotypes in model systems.
CaMKV CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Camkv gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Camkv together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Camkv open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CaMKV protein expression.
This CRISPR knockout system enables efficient generation of Camkv-deficient cell models for investigation of CaMKV signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Camkv exon(s) critical for CaMKV function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Camkv genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CaMKV CRISPR/Cas9 KO Plasmid (m) and CaMKV CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Camkv locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CaMKV HDR Plasmid (m) and CaMKV HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Camkv homology arms to support homology-directed repair at defined Camkv target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.