CaMKKα Antibody (F-2): sc-17827

CaMKKα Antibody (F-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects CaMKK alpha protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-CaMKKα antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CaMKK alpha protein plays a crucial role in cellular signaling pathways by acting as a key regulator of calcium/calmodulin-dependent protein kinases, which are essential for various physiological processes, including muscle contraction, neurotransmitter release, and gene expression. Specifically, CaMKK is responsible for phosphorylating and activating CaMKI, which subsequently influences downstream signaling cascades. This activation is particularly important in T cells, where stimulation of the T cell receptor CD3 complex can lead to a significant increase in CaMKI activity, thereby impacting immune responses. Understanding the function of CaMKK alpha and its interactions within these signaling pathways is vital for elucidating its role in health and disease, making CaMKKα (F-2) antibody an invaluable tool for researchers studying calcium signaling and its implications in various biological contexts.

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CaMKKα Antibody (F-2) References:

1. Regulation of Ca(2+)/calmodulin-dependent protein kinase kinase alpha by cAMP-dependent protein kinase: I. Biochemical analysis.  |  Okuno, S., et al. 2001. J Biochem. 130: 503-13. PMID: 11574070
2. Ca2+/calmodulin-dependent protein kinase kinase-alpha regulates skeletal muscle glucose uptake independent of AMP-activated protein kinase and Akt activation.  |  Witczak, CA., et al. 2007. Diabetes. 56: 1403-9. PMID: 17287469
3. 14-3-3 Proteins directly regulate Ca(2+)/calmodulin-dependent protein kinase kinase alpha through phosphorylation-dependent multisite binding.  |  Ichimura, T., et al. 2008. FEBS Lett. 582: 661-5. PMID: 18242179
4. A Novel Role for CAMKK1 in the Regulation of the Mesenchymal Stem Cell Secretome.  |  Dong, F., et al. 2017. Stem Cells Transl Med. 6: 1759-1766. PMID: 28688176
5. The cardiac sarcoplasmic reticulum phospholamban kinase is a distinct delta-CaM kinase isozyme.  |  Baltas, LG., et al. 1995. FEBS Lett. 373: 71-5. PMID: 7589437
6. Characterization of a Ca2+/calmodulin-dependent protein kinase cascade. Molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase.  |  Tokumitsu, H., et al. 1995. J Biol Chem. 270: 19320-4. PMID: 7642608
7. Calcium/calmodulin-dependent protein kinase II downregulates both calcineurin and protein kinase C-mediated pathways for cytokine gene transcription in human T cells.  |  Hama, N., et al. 1995. J Exp Med. 181: 1217-22. PMID: 7869038
8. G1 cell cycle arrest and apoptosis are induced in NIH 3T3 cells by KN-93, an inhibitor of CaMK-II (the multifunctional Ca2+/CaM kinase).  |  Tombes, RM., et al. 1995. Cell Growth Differ. 6: 1063-70. PMID: 8519682
9. Overexpression of Ca2+/calmodulin-dependent protein kinase II inhibits neurite outgrowth of PC12 cells.  |  Tashima, K., et al. 1996. J Neurochem. 66: 57-64. PMID: 8522989
10. Activation of Ca2+/calmodulin-dependent protein kinase (CaM-kinase) IV by CaM-kinase kinase in Jurkat T lymphocytes.  |  Park, IK. and Soderling, TR. 1995. J Biol Chem. 270: 30464-9. PMID: 8530476
11. Distribution of Ca2+/calmodulin-dependent protein kinase kinase alpha in the rat central nervous system: an immunohistochemical study.  |  Nakamura, Y., et al. 1996. Neurosci Lett. 204: 61-4. PMID: 8929978
12. Studies on the substrate specificity of Ca2+/calmodulin-dependent protein kinase kinase alpha.  |  Okuno, S., et al. 1997. J Biochem. 122: 337-43. PMID: 9378711

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CaMKKα Antibody (F-2) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-CaMKKα Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CaMKKα Antibody (F-2) Specifications

Alternate Product Name: calcium/calmodulin-dependent (CaM) kinase kinase (CAMKK1)

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-73

Epitope Target: CaMKKα

Epitope Species: rat

Product Reactivity/Detection: CaMKKα

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for CaMKKα Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin