calsequestrin 2 Antibody (E-12): sc-390999

Calsequestrin 2 Antibody (E-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects calsequestrin 2 (CSQ2) in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-calsequestrin 2 antibody (E-12) is available in non-conjugated form and conjugated to agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® dyes. CSQ2 maintains calcium homeostasis within cardiac and slow skeletal muscle cells by sequestering calcium ions in the sarcoplasmic reticulum lumen. CSQ2 is essential for muscle contraction and relaxation, maintaining the calcium gradient needed for effective excitation-contraction coupling. CSQ2 function is crucial for heart health, as dysregulation can lead to cardiac arrhythmias and other muscle-related disorders. CSQ2 interacts with ryanodine receptors, Triadin, and Junctin, which are essential for calcium signaling pathways. Anti-CSQ2 antibody (E-12) effectively detects CSQ2 across multiple species and applications, making this reagent valuable for researchers studying muscle physiology and related diseases.

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calsequestrin 2 Antibody (E-12) References:

1. A 63 kDa skeletal muscle protein associated with eye muscle inflammation in Graves' disease is identified as the calcium binding protein calsequestrin.  |  Gunji, K., et al. 1999. Autoimmunity. 29: 1-9. PMID: 10052680
2. Comparative characteristics of sarcoplasmic reticulum preparations from skeletal muscles of the ground squirrel Spermophilus undulatus, rats, and rabbits.  |  Shutova, AN., et al. 1999. Biochemistry (Mosc). 64: 1250-7. PMID: 10611529
3. Site-directed mutagenesis and deletion of three phosphorylation sites of calsequestrin of skeletal muscle sarcoplasmic reticulum. Effects on intracellular targeting.  |  Nori, A., et al. 2000. Exp Cell Res. 260: 40-9. PMID: 11010809
4. Calsequestrin, a calcium sequestering protein localized at the sarcoplasmic reticulum, is not essential for body-wall muscle function in Caenorhabditis elegans.  |  Cho, JH., et al. 2000. J Cell Sci. 113 (Pt 22): 3947-58. PMID: 11058082
5. The asp-rich region at the carboxyl-terminus of calsequestrin binds to Ca(2+) and interacts with triadin.  |  Shin, DW., et al. 2000. FEBS Lett. 486: 178-82. PMID: 11113462
6. Targeting of calsequestrin to the sarcoplasmic reticulum of skeletal muscle upon deletion of its glycosylation site.  |  Nori, A., et al. 2001. Exp Cell Res. 265: 104-13. PMID: 11281648
7. Calsequestrin 2 (CASQ2) mutations increase expression of calreticulin and ryanodine receptors, causing catecholaminergic polymorphic ventricular tachycardia.  |  Song, L., et al. 2007. J Clin Invest. 117: 1814-23. PMID: 17607358
8. Calsequestrin 2 deletion causes sinoatrial node dysfunction and atrial arrhythmias associated with altered sarcoplasmic reticulum calcium cycling and degenerative fibrosis within the mouse atrial pacemaker complex1.  |  Glukhov, AV., et al. 2015. Eur Heart J. 36: 686-97. PMID: 24216388
9. An improved method for the isolation of rat cardiac sarcoplasmic reticulum.  |  Barker, PA., et al. 1988. Mol Cell Biochem. 84: 87-95. PMID: 2976479
10. Calsequestrin 2 overexpression in breast cancer increases tumorigenesis and metastasis by modulating the tumor microenvironment.  |  Kim, JH., et al. 2022. Mol Oncol. 16: 466-484. PMID: 34743414
11. Monomorphic and Polymorphic Ventricular Arrhythmias in Heterozygous Calsequestrin-2 Mutation Carriers.  |  Jiménez-Jáimez, J., et al. 2022. Circ Genom Precis Med. 15: e003518. PMID: 35119302
12. The function and regulation of calsequestrin-2: implications in calcium-mediated arrhythmias.  |  Sibbles, ET., et al. 2022. Biophys Rev. 14: 329-352. PMID: 35340602

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-calsequestrin 2 Antibody (E-12) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-calsequestrin 2 Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

calsequestrin 2 Antibody (E-12) Specifications

Alternate Product Name: calsequestrin 2

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 21-80

Epitope Target: calsequestrin 2

Epitope Species: human

Product Reactivity/Detection: calsequestrin 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for calsequestrin 2 Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin