Calpain reg Antibody (P-1): sc-32325

Calpain reg Antibody (P-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Calpain reg protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Calpain reg antibody (P-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Calpain reg protein plays a crucial role as a regulatory subunit in the calpain family of calcium-dependent cysteine proteases, which are essential for mediating specific calcium-dependent cellular processes such as cell fusion, mitosis, and meiosis. These proteases are heterodimers composed of a small regulatory subunit, Calpain reg, and one of three large catalytic subunits: Calpain 1, Calpain 2, or Calpain p94. The regulation of calpain activity is influenced by calpastatin, which inhibits both proteolytic activity of calpain and membrane binding. This regulatory mechanism is particularly important in various physiological processes, including spermatogenesis and the acrosome reaction, which precedes fertilization. Additionally, Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain, highlighting potential involvement in neuroinflammatory responses. Calpain reg (P-1) antibody effectively detects and characterizes Calpain reg protein, making this antibody an invaluable tool for researchers studying the intricate roles of calpains in cellular signaling and pathology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Calpain reg Antibody (P-1) References:

1. Activation of calpain in myocardial infarction: an immunohistochemical study using a calpain antibody raised against active site histidine-containing peptide.  |  Kunimatsu, M., et al. 1999. Cardiovasc Pathol. 8: 7-15. PMID: 10722243
2. Molecular diversity of erythrocyte calpastatin.  |  Takano, E., et al. 1991. Biomed Biochim Acta. 50: 517-21. PMID: 1801717
3. Calpain-1 contributes to IgE-mediated mast cell activation.  |  Wu, Z., et al. 2014. J Immunol. 192: 5130-9. PMID: 24760147
4. Extracellular CIRP Induces Calpain Activation in Neurons via PLC-IP3-Dependent Calcium Pathway.  |  Sharma, A., et al. 2023. Mol Neurobiol. 60: 3311-3328. PMID: 36853429
5. Focal adhesion kinase activation by calcium-dependent calpain is involved in chronic lymphocytic leukaemia cell aggressiveness.  |  Severin, F., et al. 2023. Br J Haematol. 203: 224-236. PMID: 37495265
6. Development of a monoclonal antibody specific for a calpain-generated ∆48 kDa calcineurin fragment, a marker of distressed astrocytes.  |  Kraner, SD., et al. 2024. J Neurosci Methods. 402: 110012. PMID: 37984591
7. Antibody conjugated targeted nanotherapy epigenetically inhibits calpain-mediated mitochondrial dysfunction to attenuate Parkinson's disease.  |  Biswal, L., et al. 2024. Carbohydr Polym. 346: 122575. PMID: 39245478
8. Calcium-dependent proteinases and specific inhibitors: calpain and calpastatin.  |  Murachi, T. 1984. Biochem Soc Symp. 49: 149-67. PMID: 6100833
9. Molecular diversity of calpastatin in human erythroid cells.  |  Takano, E., et al. 1993. Arch Biochem Biophys. 303: 349-54. PMID: 8512320
10. Autolysis, Ca2+ requirement, and heterodimer stability in m-calpain.  |  Elce, JS., et al. 1997. J Biol Chem. 272: 11268-75. PMID: 9111030
11. Regulation of the calpain-calpastatin system by membranes (review).  |  Kawasaki, H. and Kawashima, S. 1996. Mol Membr Biol. 13: 217-24. PMID: 9116760
12. Calpains: intact and active?  |  Johnson, GV. and Guttmann, RP. 1997. Bioessays. 19: 1011-8. PMID: 9394623

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Calpain reg Antibody (P-1) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Calpain reg Antibody (P-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Calpain reg Antibody (P-1) Specifications

Alternate Product Name: CAPNS1

Product Clone Name: P-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Calpain reg

Epitope Species: bovine

Product Reactivity/Detection: Calpain reg

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Calpain reg Antibody (P-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin