Calpain 9 Antibody (G-12): sc-166517
- Calpain 9 Antibody (G-12) is a mouse monoclonal IgG2b κ Calpain 9 antibody provided at 200 µg/ml
- raised against amino acids 481-690 mapping at the C-terminus of Calpain 9 of human origin
- Calpain 9 Antibody (G-12) is recommended for detection of Calpain 9 of human origin by WB, IP, IF and ELISA
- Anti-Calpain 9 Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Calpain 9 Antibody (G-12) for WB applications. These reagents are now offered in bundles with Calpain 9 Antibody (G-12) (see ordering information below).
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Calpain 9 Antibody (G-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects Calpain 9 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Calpain 9 (G-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Calpain 9, a member of the calpain family, is a calcium-activated cysteine protease that plays a crucial role in cellular processes such as apoptosis, cell migration, and cytoskeletal remodeling. Calpain 9′s localization within the cytoplasm allows for the regulation of various signaling pathways essential for maintaining cellular homeostasis. Calpain 9 has been implicated in gastric cancer pathology, where downregulation in gastric cancer cell lines suggests a potential tumor-suppressive function. Calpain 9 exists in two isoforms due to alternative splicing, which may contribute to functional diversity and regulatory mechanisms in different tissues. Understanding Calpain 9′s role in these contexts is vital for developing therapeutic strategies targeting related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Calpain 9 Antibody (G-12) References:
- The calpain family and human disease. | Huang, Y. and Wang, KK. 2001. Trends Mol Med. 7: 355-62. PMID: 11516996
- Down-regulation of calpain 9 is linked to hypertensive heart and kidney disease. | Markmann, A., et al. 2005. Cell Physiol Biochem. 15: 109-16. PMID: 15665521
- The crystal structures of human calpains 1 and 9 imply diverse mechanisms of action and auto-inhibition. | Davis, TL., et al. 2007. J Mol Biol. 366: 216-29. PMID: 17157313
- Role of calpain-9 and PKC-delta in the apoptotic mechanism of lumen formation in CEACAM1 transfected breast epithelial cells. | Chen, CJ., et al. 2010. Exp Cell Res. 316: 638-48. PMID: 19909740
- Low calpain-9 is associated with adverse disease-specific survival following endocrine therapy in breast cancer. | Davis, J., et al. 2014. BMC Cancer. 14: 995. PMID: 25539577
- Decreased expression of Calpain-9 predicts unfavorable prognosis in patients with gastric cancer. | Peng, P., et al. 2016. Sci Rep. 6: 29604. PMID: 27404891
- Calpain 9 as a therapeutic target in TGFβ-induced mesenchymal transition and fibrosis. | Kim, DH., et al. 2019. Sci Transl Med. 11: PMID: 31316008
- MiR-585-3p suppresses tumor proliferation and migration by directly targeting CAPN9 in high grade serous ovarian cancer. | Lu, X., et al. 2021. J Ovarian Res. 14: 90. PMID: 34238324
- Calcium-dependent proteinases and specific inhibitors: calpain and calpastatin. | Murachi, T. 1984. Biochem Soc Symp. 49: 149-67. PMID: 6100833
- Regulation of the calpain-calpastatin system by membranes (review). | Kawasaki, H. and Kawashima, S. 1996. Mol Membr Biol. 13: 217-24. PMID: 9116760
- Calpains: intact and active? | Johnson, GV. and Guttmann, RP. 1997. Bioessays. 19: 1011-8. PMID: 9394623
- Characterization of a human digestive tract-specific calpain, nCL-4, expressed in the baculovirus system. | Lee, HJ., et al. 1999. Arch Biochem Biophys. 362: 22-31. PMID: 9917325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calpain 9 Antibody (G-12) | sc-166517 | 200 µg/ml | $322.00 | |||
Calpain 9 Antibody (G-12): m-IgGκ BP-HRP Bundle | sc-521638 | 200 µg Ab, 40 µg BP | $361.00 | |||
Calpain 9 Antibody (G-12): m-IgG2b BP-HRP Bundle | sc-548944 | 200 µg Ab; 10 µg BP | $361.00 | |||
Calpain 9 Antibody (G-12) AC | sc-166517 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Calpain 9 Antibody (G-12) HRP | sc-166517 HRP | 200 µg/ml | $322.00 | |||
Calpain 9 Antibody (G-12) FITC | sc-166517 FITC | 200 µg/ml | $336.00 | |||
Calpain 9 Antibody (G-12) PE | sc-166517 PE | 200 µg/ml | $349.00 | |||
Calpain 9 Antibody (G-12) Alexa Fluor® 488 | sc-166517 AF488 | 200 µg/ml | $364.00 | |||
Calpain 9 Antibody (G-12) Alexa Fluor® 546 | sc-166517 AF546 | 200 µg/ml | $364.00 | |||
Calpain 9 Antibody (G-12) Alexa Fluor® 594 | sc-166517 AF594 | 200 µg/ml | $364.00 | |||
Calpain 9 Antibody (G-12) Alexa Fluor® 647 | sc-166517 AF647 | 200 µg/ml | $364.00 | |||
Calpain 9 Antibody (G-12) Alexa Fluor® 680 | sc-166517 AF680 | 200 µg/ml | $364.00 | |||
Calpain 9 Antibody (G-12) Alexa Fluor® 790 | sc-166517 AF790 | 200 µg/ml | $364.00 |