Calpain 13 Antibody (A-4): sc-514082
- Calpain 13 Antibody (A-4) is a mouse monoclonal IgG2a κ Calpain 13 antibody provided at 200 µg/ml
- raised against amino acids 366-665 mapping at the C-terminus of Calpain 13 of mouse origin
- Calpain 13 Antibody (A-4) is recommended for detection of Calpain 13 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-Calpain 13 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Calpain 13 Antibody (A-4) for WB applications. These reagents are now offered in bundles with Calpain 13 Antibody (A-4) (see ordering information below).
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Calpain 13 Antibody (A-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Calpain 13 (CAPN13) of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CAPN13 antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CAPN13, also known as Sol H, is a unique member of the calpain family, characterized by its divergence from other calpains. CAPN13 plays a crucial role in intracellular protein processing, which is essential for various cellular functions, including cell cycle regulation and apoptosis. CAPN13 is composed of four distinct domains and consists of 423 amino acid residues, with a limited tissue distribution primarily found in the human brain, testis, and lung. Notably, CAPN13 is a homolog of the Drosophila optic lobe gene product Sol, highlighting its evolutionary significance. CAPN13′s structure, which allows for the formation of heterodimers with varying large subunits, is vital for enzymatic activity and functional versatility. CAPN13′s ability to operate independently of its small subunit further emphasizes its importance in cellular signaling pathways and proteolytic processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Calpain 13 Antibody (A-4) References:
- Diverse mRNA expression patterns of the mouse calpain genes Capn5, Capn6 and Capn11 during development. | Dear, TN. and Boehm, T. 1999. Mech Dev. 89: 201-9. PMID: 10559499
- The calpain family and human disease. | Huang, Y. and Wang, KK. 2001. Trends Mol Med. 7: 355-62. PMID: 11516996
- Identification and characterization of two novel calpain large subunit genes. | Dear, TN. and Boehm, T. 2001. Gene. 274: 245-52. PMID: 11675017
- Structure, activation, and biology of calpain. | Suzuki, K., et al. 2004. Diabetes. 53 Suppl 1: S12-8. PMID: 14749260
- Inhibition of calpain cleavage of huntingtin reduces toxicity: accumulation of calpain/caspase fragments in the nucleus. | Gafni, J., et al. 2004. J Biol Chem. 279: 20211-20. PMID: 14981075
- Calpain-cleaved collapsin response mediator protein-3 induces neuronal death after glutamate toxicity and cerebral ischemia. | Hou, ST., et al. 2006. J Neurosci. 26: 2241-9. PMID: 16495451
- Calpain 11 is unique to mouse spermatogenic cells. | Ben-Aharon, I., et al. 2006. Mol Reprod Dev. 73: 767-73. PMID: 16541461
- The therapeutic potential of the calpain family: new aspects. | Saez, ME., et al. 2006. Drug Discov Today. 11: 917-23. PMID: 16997142
- Cerebral calpain in fatal falciparum malaria. | Medana, IM., et al. 2007. Neuropathol Appl Neurobiol. 33: 179-92. PMID: 17359359
- Calpeptin, not calpain, directly inhibits an ion channel of the inner mitochondrial membrane. | Derksen, M., et al. 2016. Protoplasma. 253: 835-843. PMID: 26108743
- Mitochondrial localization of calpain-13 in mouse brain. | Funajima, E., et al. 2022. Biochem Biophys Res Commun. 609: 149-155. PMID: 35429682
- Proteolysis of mitochondrial calpain-13 in cerebral ischemia-reperfusion injury. | Chukai, Y., et al. 2024. Biochem Biophys Rep. 39: 101768. PMID: 39050013
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calpain 13 Antibody (A-4) | sc-514082 | 200 µg/ml | $316.00 | |||
Calpain 13 Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-531024 | 200 µg Ab; 10 µg BP | $354.00 | |||
Calpain 13 Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-524599 | 200 µg Ab, 40 µg BP | $354.00 | |||
Calpain 13 Antibody (A-4): m-IgG2a BP-HRP Bundle | sc-547666 | 200 µg Ab; 10 µg BP | $354.00 | |||
Calpain 13 Antibody (A-4) AC | sc-514082 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Calpain 13 Antibody (A-4) HRP | sc-514082 HRP | 200 µg/ml | $316.00 | |||
Calpain 13 Antibody (A-4) FITC | sc-514082 FITC | 200 µg/ml | $330.00 | |||
Calpain 13 Antibody (A-4) PE | sc-514082 PE | 200 µg/ml | $343.00 | |||
Calpain 13 Antibody (A-4) Alexa Fluor® 488 | sc-514082 AF488 | 200 µg/ml | $357.00 | |||
Calpain 13 Antibody (A-4) Alexa Fluor® 546 | sc-514082 AF546 | 200 µg/ml | $357.00 | |||
Calpain 13 Antibody (A-4) Alexa Fluor® 594 | sc-514082 AF594 | 200 µg/ml | $357.00 | |||
Calpain 13 Antibody (A-4) Alexa Fluor® 647 | sc-514082 AF647 | 200 µg/ml | $357.00 | |||
Calpain 13 Antibody (A-4) Alexa Fluor® 680 | sc-514082 AF680 | 200 µg/ml | $357.00 | |||
Calpain 13 Antibody (A-4) Alexa Fluor® 790 | sc-514082 AF790 | 200 µg/ml | $357.00 |