Calpain 1 Antibody (D-11): sc-271313

Calpain 1 Antibody (D-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Calpain 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Calpain 1 antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Calpain 1, also known as µ-calpain, is an intracellular calcium-dependent protease that plays a crucial role in cleaving cytoskeletal and submembranous proteins, which is essential for maintaining cellular structure and function. Calpain 1 is primarily located in the cytoplasm and is activated by calcium ions, allowing participation in various cellular processes such as cell cycle regulation, apoptosis, and signal transduction. Proper functioning of Calpain 1 is vital for processes like cell fusion and spermatogenesis, as Calpain 1 influences the acrosome reaction, a key event in fertilization. Dysregulation of Calpain 1 activity has been implicated in several pathological conditions, including neurodegenerative diseases and cancer, making Calpain 1 a significant target for therapeutic intervention. Calpain 1 (D-11) antibody is an invaluable tool for researchers studying these critical biological processes and the role of Calpain 1 in health and disease.

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Calpain 1 Antibody (D-11) References:

1. Calpain-1-dependent degradation of troponin I mutants found in familial hypertrophic cardiomyopathy.  |  Barta, J., et al. 2003. Mol Cell Biochem. 251: 83-8. PMID: 14575308
2. Calpain-1 regulates Bax and subsequent Smac-dependent caspase-3 activation in neutrophil apoptosis.  |  Altznauer, F., et al. 2004. J Biol Chem. 279: 5947-57. PMID: 14612448
3. The expression of calpain 1 and calpain 2 in spermatogenic cells and spermatozoa of the mouse.  |  Ben-Aharon, I., et al. 2005. Reproduction. 129: 435-42. PMID: 15798018
4. Molecular diversity of erythrocyte calpastatin.  |  Takano, E., et al. 1991. Biomed Biochim Acta. 50: 517-21. PMID: 1801717
5. Calpain 1 cleaves and inactivates prostacyclin synthase in mesenteric arteries from diabetic mice.  |  Randriamboavonjy, V., et al. 2017. Basic Res Cardiol. 112: 10. PMID: 28013348
6. Calpain-2 specifically cleaves Junctophilin-2 at the same site as Calpain-1 but with less efficacy.  |  Wang, J., et al. 2021. Biochem J. 478: 3539-3553. PMID: 34524407
7. Calpain-1 inhibition attenuates in vivo thrombosis in a humanized model of sickle cell disease.  |  Mithila, F., et al. 2022. Thromb Res. 211: 123-126. PMID: 35149397
8. Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway.  |  Zhao, F., et al. 2023. J Ginseng Res. 47: 144-154. PMID: 36644390
9. Calcium-dependent proteinases and specific inhibitors: calpain and calpastatin.  |  Murachi, T. 1984. Biochem Soc Symp. 49: 149-67. PMID: 6100833
10. Molecular diversity of calpastatin in human erythroid cells.  |  Takano, E., et al. 1993. Arch Biochem Biophys. 303: 349-54. PMID: 8512320
11. Regulation of the calpain-calpastatin system by membranes (review).  |  Kawasaki, H. and Kawashima, S. 1996. Mol Membr Biol. 13: 217-24. PMID: 9116760
12. Calpains: intact and active?  |  Johnson, GV. and Guttmann, RP. 1997. Bioessays. 19: 1011-8. PMID: 9394623

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Calpain 1 Antibody (D-11) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-Calpain 1 Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Calpain 1 Antibody (D-11) Specifications

Alternate Product Name: CAPN1

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 8-33

Epitope Target: Calpain 1

Epitope Species: human

Product Reactivity/Detection: Calpain 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for Calpain 1 Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin