Calpain 1 Antibody (5B3): sc-32796

Calpain 1 Antibody (5B3) is a mouse monoclonal IgG2a antibody that detects Calpain 1 in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Calpain 1, also known as µ-calpain, is an intracellular calcium-dependent protease that plays a crucial role in various cellular processes by cleaving cytoskeletal and submembranous proteins. Anti-Calpain 1 antibody (5B3) recognizes a protein essential for mediating specific calcium-dependent functions, including cell fusion, mitosis, and meiosis, which are vital for normal cellular function and development. The structure of Calpain 1 is characterized by its heterodimeric composition, consisting of a small regulatory subunit and a large catalytic subunit, which allows for precise regulation of proteolytic activity. Calpain 1′s regulation is further influenced by calpastatin, which inhibits both activity and binding to membranes, highlighting the importance of post-translational modifications in controlling function. Notably, Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain, suggesting involvement in neuroinflammatory processes. Calpain 1 is critical in spermatogenesis and the acrosome reaction, underscoring significance in reproductive biology. Calpain 1 (5B3) antibody serves as an invaluable tool for researchers studying cellular dynamics and signaling pathways.

The preferred Calpain 1 antibody is Calpain 1 Antibody (D-11): sc-271313.