Caldesmon Antibody (8) recommended for detection of H-Caldesmon and L-Caldesmon of mouse, rat and human origin by WB and IF

Caldesmon Antibody (8): sc-135965

Caldesmon Antibody (8) is rated 4.0 out of 5 by 2.
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Datasheets

    • Caldesmon Antibody (8) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 251-395 of L-Caldesmon of human origin
    • recommended for detection of H-Caldesmon and L-Caldesmon of mouse, rat and human origin by WB and IF; not recommended for IP
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What is the recommended fixation method for immunofluorescence staining with Caldesmon (8): sc-135965 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good specificity and signal strength in western Good specificity and signal strength in western blot using Hs68 tissue extract. -SCBT QC
Date published: 2015-02-03
Rated 4 out of 5 by from Good immunofluorescence cytoplasmic and membrane Good immunofluorescence cytoplasmic and membrane staining in WI-38 cells. -SCBT QC
Date published: 2015-01-08
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