Calcyclin Antibody (F-1): sc-271396

Calcyclin Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Calcyclin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Calcyclin antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Calcyclin, also known as Prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9, S-100 calcium-binding protein A6 (S-100A6), or MLN 4, is a homodimeric member of the S-100 calcium-binding protein family, which plays a crucial role in calcium signaling pathways. Calcyclin is primarily located in the cytoplasm and nucleus of cells, where Calcyclin is involved in regulating various cellular processes such as growth, proliferation, and secretion. This localization allows for rapid responses to calcium fluctuations, which are essential for processes like muscle contraction, neurotransmitter release, and cell differentiation. Calcyclin is upregulated in proliferating and differentiating cells and is often overexpressed in acute myeloid leukemias, highlighting Calcyclin′s potential as a biomarker for certain cancers. Calcyclin interacts with S-100B protein, with binding being stabilized by calcium and zinc, and associates with Annexin XI and CacyBP (calcyclin-binding protein), further emphasizing Calcyclin′s importance in cellular signaling and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Calcyclin Antibody (F-1) References:

1. Calcyclin mediates serum response element (SRE) activation by an osteoblastic extracellular cation-sensing mechanism.  |  Tu, Q., et al. 2003. J Bone Miner Res. 18: 1825-33. PMID: 14584893
2. Involvement of S100A6 (calcyclin) and its binding partners in intracellular signaling pathways.  |  Filipek, A., et al. 2008. Adv Enzyme Regul. 48: 225-39. PMID: 18155169
3. Calcyclin-like protein from Ehrlich ascites tumour cells-Ca2(+)-binding properties, distribution and target protein.  |  Kuźnicki, J. and Filipek, A. 1990. Adv Exp Med Biol. 269: 149-52. PMID: 2191553
4. Calcyclin binding protein and Siah-1 interacting protein in Alzheimer's disease pathology: neuronal localization and possible function.  |  Wasik, U., et al. 2013. Neurobiol Aging. 34: 1380-8. PMID: 23260124
5. Calcyclin Binding Protein/Siah-1 Interacting Protein Is a Hsp90 Binding Chaperone.  |  Góral, A., et al. 2016. PLoS One. 11: e0156507. PMID: 27249023
6. Quantification of Calcyclin and Heat Shock Protein 90 in Sera from Women with and without Preeclampsia by Mass Spectrometry.  |  Güzel, C., et al. 2019. Proteomics Clin Appl. 13: e1800181. PMID: 30417587
7. Heterozygous calcyclin-binding protein/Siah1-interacting protein (CACYBP/SIP) gene pathogenic variant linked to a dominant family with paucity of interlobular bile duct.  |  Kanno, M., et al. 2022. J Hum Genet. 67: 393-397. PMID: 35087201
8. Protective effects of calcyclin-binding protein against pulmonary vascular remodeling in flow-associated pulmonary arterial hypertension.  |  Zhou, J., et al. 2022. Respir Res. 23: 223. PMID: 36042446
9. Biochemical properties of calcyclin--a potential marker of some diseases.  |  Wojda, U. and Kuźnicki, J. 1993. Acta Biochim Pol. 40: 171-5. PMID: 8372551
10. Calcyclin--Ca(2+)-binding protein homologous to glial S-100 beta is present in neurones.  |  Filipek, A., et al. 1993. Neuroreport. 4: 383-6. PMID: 8499593

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Calcyclin Antibody (F-1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Calcyclin Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Calcyclin Antibody (F-1) Specifications

Alternate Product Name: Calcyclin

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 36-90

Epitope Target: Calcyclin

Epitope Species: human

Product Reactivity/Detection: Calcyclin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Calcyclin Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Calcyclin antibody is Calcyclin Antibody (7D11A8): sc-53950.