CAF-1 p60 Antibody (SS 53): sc-56646
- CAF-1 p60 Antibody (SS 53) is a mouse monoclonal IgG1 κ CAF-1 p60 antibody provided at 200 µg/ml
- raised against full length CAF-1 p60 of human origin
- CAF-1 p60 Antibody (SS 53) is recommended for detection of CAF-1 p60 of mouse, rat and human origin by WB, IP and ELISA
- Anti-CAF-1 p60 Antibody (SS 53) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CAF-1 p60 Antibody (SS 53) for WB applications. These reagents are now offered in bundles with CAF-1 p60 Antibody (SS 53) (see ordering information below).
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CAF-1 p60 Antibody (SS 53) is a mouse monoclonal IgG1 kappa light chain antibody that detects CAF-1 p60 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-CAF-1 p60 antibody (SS 53) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The chromatin assembly factor-1 (CAF-1) complex, which includes the p60 subunit, plays a crucial role in the assembly of nucleosomes by depositing newly synthesized and acetylated histones H3 and H4 into nascent chromatin during DNA replication. This process is vital for maintaining genomic integrity and regulating gene expression, as proper nucleosome assembly is essential for the accessibility of DNA to transcription factors and other regulatory proteins. Additionally, the p60 subunit is involved in dynamic interactions with heterochromatin, which is characterized by its tightly packed structure that generally replicates late in the S phase of the cell cycle. The ability of CAF-1 to facilitate the orderly assembly of histones and heterochromatin proteins during DNA replication underscores its importance in cellular processes such as DNA repair, replication, and gene regulation. CAF-1 p60 monoclonal antibody (SS 53) enables researchers to gain insights into the mechanisms of chromatin dynamics and the role of CAF-1 in cellular function and development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CAF-1 p60 Antibody (SS 53) References:
- Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells. | Minc, E., et al. 1999. Chromosoma. 108: 220-34. PMID: 10460410
- Heterochromatin dynamics in mouse cells: interaction between chromatin assembly factor 1 and HP1 proteins. | Murzina, N., et al. 1999. Mol Cell. 4: 529-40. PMID: 10549285
- Duplication and maintenance of heterochromatin domains. | Taddei, A., et al. 1999. J Cell Biol. 147: 1153-66. PMID: 10601331
- Identification of heterochromatin protein 1 (HP1) as a phosphorylation target by Pim-1 kinase and the effect of phosphorylation on the transcriptional repression function of HP1(1). | Koike, N., et al. 2000. FEBS Lett. 467: 17-21. PMID: 10664448
- Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro. | Smith, S. and Stillman, B. 1989. Cell. 58: 15-25. PMID: 2546672
- The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication. | Kaufman, PD., et al. 1995. Cell. 81: 1105-14. PMID: 7600578
- Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4. | Verreault, A., et al. 1996. Cell. 87: 95-104. PMID: 8858152
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CAF-1 p60 Antibody (SS 53) | sc-56646 | 200 µg/ml | $322.00 | |||
CAF-1 p60 Antibody (SS 53): m-IgG Fc BP-HRP Bundle | sc-526884 | 200 µg Ab; 10 µg BP | $361.00 | |||
CAF-1 p60 Antibody (SS 53): m-IgG1 BP-HRP Bundle | sc-532257 | 200 µg Ab; 20 µg BP | $361.00 | |||
CAF-1 p60 Antibody (SS 53) AC | sc-56646 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CAF-1 p60 Antibody (SS 53) HRP | sc-56646 HRP | 200 µg/ml | $322.00 | |||
CAF-1 p60 Antibody (SS 53) FITC | sc-56646 FITC | 200 µg/ml | $336.00 | |||
CAF-1 p60 Antibody (SS 53) PE | sc-56646 PE | 200 µg/ml | $349.00 | |||
CAF-1 p60 Antibody (SS 53) Alexa Fluor® 488 | sc-56646 AF488 | 200 µg/ml | $364.00 | |||
CAF-1 p60 Antibody (SS 53) Alexa Fluor® 546 | sc-56646 AF546 | 200 µg/ml | $364.00 | |||
CAF-1 p60 Antibody (SS 53) Alexa Fluor® 594 | sc-56646 AF594 | 200 µg/ml | $364.00 | |||
CAF-1 p60 Antibody (SS 53) Alexa Fluor® 647 | sc-56646 AF647 | 200 µg/ml | $364.00 | |||
CAF-1 p60 Antibody (SS 53) Alexa Fluor® 680 | sc-56646 AF680 | 200 µg/ml | $364.00 | |||
CAF-1 p60 Antibody (SS 53) Alexa Fluor® 790 | sc-56646 AF790 | 200 µg/ml | $364.00 |