CAF-1 p150 Antibody (SS 1 1-13): sc-56643

CAF-1 p150 Antibody (SS 1 1-13) is a mouse monoclonal IgG1 antibody that detects chromatin assembly factor-1 p150 (CAF-1 p150) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). CAF-1 p150 is a crucial component of the chromatin assembly factor-1 complex, which plays a vital role in the deposition of newly synthesized and acetylated histones H3 and H4 into nascent chromatin during DNA replication. This process is essential for maintaining the structural integrity of the genome, as DNA is properly packaged into chromatin, allowing for accurate replication and gene expression. The p150 subunit is particularly important for the maintenance of heterochromatin, which is characterized by its densely coiled structure and low gene density, making heterochromatin relatively inaccessible to DNA-modifying enzymes. During late S phase, p150 interacts directly with heterochromatin-associated proteins, such as HP1α, HP1β, and HP1γ, facilitating the orderly assembly of chromatin. As cells transition into mitosis, the dissociation of p150 and HP1 proteins from heterochromatin occurs alongside the phosphorylation of histone H3, highlighting the dynamic nature of chromatin organization. CAF-1 p150′s ability to regulate these processes underscores its significance in cellular function and genomic stability, making anti-CAF-1 p150 antibody (SS 1 1-13) an invaluable tool for researchers studying chromatin dynamics and epigenetic regulation.

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CAF-1 p150 Antibody (SS 1 1-13) References:

1. Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene.  |  Dong, H., et al. 2001. Gene. 264: 187-96. PMID: 11250073
2. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1.  |  Reese, BE., et al. 2003. Mol Cell Biol. 23: 3226-36. PMID: 12697822
3. The replication kinase Cdc7-Dbf4 promotes the interaction of the p150 subunit of chromatin assembly factor 1 with proliferating cell nuclear antigen.  |  Gérard, A., et al. 2006. EMBO Rep. 7: 817-23. PMID: 16826239
4. Gene-specific transcriptional activation mediated by the p150 subunit of the chromatin assembly factor 1.  |  Lee, SB., et al. 2009. J Biol Chem. 284: 14040-9. PMID: 19324875
5. The p150 subunit of CAF-1 causes association of SUMO2/3 with the DNA replication foci.  |  Uwada, J., et al. 2010. Biochem Biophys Res Commun. 391: 407-13. PMID: 19919826
6. The p150 subunit of the histone chaperone Caf-1 interacts with the transcriptional repressor Gfi1.  |  Heyd, F., et al. 2011. Biochim Biophys Acta. 1809: 255-61. PMID: 21570500
7. A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli.  |  Smith, CL., et al. 2014. Mol Biol Cell. 25: 2866-81. PMID: 25057015
8. The role of the chromatin assembly complex (CAF-1) and its p60 subunit (CHAF1b) in homeostasis and disease.  |  Volk, A. and Crispino, JD. 2015. Biochim Biophys Acta. 1849: 979-86. PMID: 26066981
9. Chromatin Assembly Factor 1 (CAF-1) facilitates the establishment of facultative heterochromatin during pluripotency exit.  |  Cheng, L., et al. 2019. Nucleic Acids Res. 47: 11114-11131. PMID: 31586391
10. Unorthodox PCNA Binding by Chromatin Assembly Factor 1.  |  Gopinathan Nair, A., et al. 2022. Int J Mol Sci. 23: PMID: 36232396

The preferred CAF-1 p150 antibody is CAF-1 p150 Antibody (D-1): sc-133105.