Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
cadherin-19 CRISPR/Cas9 KO Plasmid (h): sc-401716
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cadherin-19 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the cadherin-19 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cadherin-19 Antibody (D-1): sc-376990
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cadherin-19 CRISPR/Cas9 KO Plasmid (h) | sc-401716 | 20 µg | $397.00 |
Overview
CDH19 encodes cadherin-19, a calcium-dependent cell–cell adhesion molecule that contributes to tissue architecture and contact-mediated signaling. As a classical cadherin family member, cadherin-19 supports adherens junction organization via interactions with catenins and linkage to the actin cytoskeleton, influencing processes such as cell polarity, migration, and differentiation. CDH19 expression has been reported in neural and glial lineages, where cadherin-mediated adhesion can shape developmental patterning and cellular interactions. Dysregulated cadherin expression and junctional remodeling are frequently associated with altered invasiveness and lineage identity in disease contexts, making CDH19 a useful target for studying adhesion-dependent phenotypes.
cadherin-19 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDH19 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDH19 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDH19 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish cadherin-19 protein expression.
This CRISPR knockout system enables efficient generation of CDH19-deficient cell models for investigation of cadherin-19 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDH19 exon(s) critical for cadherin-19 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDH19 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by cadherin-19 CRISPR/Cas9 KO Plasmid (h) and cadherin-19 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDH19 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by cadherin-19 HDR Plasmid (h) and cadherin-19 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDH19 homology arms to support homology-directed repair at defined CDH19 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.