Cables1 Antibody (D-10) is a mouse monoclonal IgG2a κ Cables1 antibody provided at 200 µg/ml
raised against amino acids 1-280 mapping at the N-terminus of Cables1 of mouse origin
Cables1 Antibody (D-10) is recommended for detection of Cables1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-Cables1 Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cables1 (D-10): sc-374316.
m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cables1 Antibody (D-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Cables1 Antibody (D-10) (see ordering information below).
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Cables1 Antibody (D-10) is an IgG2a κ mouse monoclonal Cables1 antibody (also designated Cables1 antibody) suitable for the detection of the Cables1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Cables1 Antibody (D-10) is available as both the non-conjugated anti-Cables1 antibody form, as well as multiple conjugated forms of anti-Cables1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Normal Abl function is essential for humans because Philadelphia chromosome translocation involving the ABL gene causes chronic myelogenous leu-kemia. Abl associates with a broad range of targets and appears to function in various signaling pathway. Cables1, a 568 amino acid protein, links Abl to cyclin-dependent kinase 5 (Cdk5). Cables1 bound to Cdk5 functions as a substrate for phosphorylation by the Cdk5/p35 kinase. Cables contains an area of weak homology to cyclin A and cyclin C. In addition to its C-terminal Cdk5 binding domain, Cables1 also has six potential SH3 binding motifs (PXXP) clustered around the amino-terminus, two of which are similar to motifs known to bind the Abl SH3 domain. Cables1 forms a trimolecular complex with Cdk5 and Abl in vivo. All three proteins co-localize within cortical axons, particularly in their growth cones. Cables1 and Abl may function as adaptor or scaffolding proteins to bind to Cdk5 and control its subcellular location in the neuron.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz® conjugates which eliminates the need for a secondary antibody.
Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cables1 Antibody (D-10) has 0 citations in a variety of scientific publications.
Primary antibodies like Anti-Cables1 Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.
Cables1 Antibody (D-10) Specifications
Alternate Product Name
Product Clone Name
IgG2a kappa light chain
mouse, rat, human
Additional Mammalian Reactivity
Additional Non-Mammalian Reactivity
WB, IP, IF, IHC(P), ELISA
Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin
0 citations in various scientific publications
Recommended Secondary Detection Reagents
Mouse IgG2a Binding Proteins
m-IgG2a BP-HRP: sc-542731 (Mouse IgG2a binding protein conjugated to horseradish peroxidase)
m-IgG2a BP-B: sc-542734 (Mouse IgG2a binding protein conjugated to biotin)
m-IgG2a BP-FITC: sc-542733 (Mouse IgG2a binding protein conjugated to fluorescein isothiocyanate)
Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.
Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-Cables1 Antibody (D-10) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).
Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.
Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.
Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.
Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.
Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.
I am using Cables1 (D-10): sc-374316 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?
Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
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