Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CAB39L CRISPR/Cas9 KO Plasmid (h): sc-416543
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CAB39L CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CAB39L genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CAB39L Antibody (Q32): sc-100390
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CAB39L CRISPR/Cas9 KO Plasmid (h) | sc-416543 | 20 µg | $397.00 |
Overview
CAB39L encodes calcium-binding protein 39-like, a conserved adaptor that associates with the serine/threonine kinase STK11/LKB1 and promotes formation and function of the LKB1–STRAD–CAB39 scaffold complex. Through this complex, CAB39L supports activation of AMPK family kinases, linking cellular energy status to metabolic control, stress responses, and polarity programs. CAB39L-dependent signaling influences processes such as mitochondrial homeostasis, autophagy regulation, and epithelial organization via AMPK-related pathways. Dysregulation of LKB1-AMPK axis components is implicated in cancer biology and metabolic disease mechanisms, making CAB39L a useful node for interrogating pathway wiring and context-specific vulnerabilities.
CAB39L CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CAB39L gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CAB39L together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CAB39L open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CAB39L protein expression.
This CRISPR knockout system enables efficient generation of CAB39L-deficient cell models for investigation of CAB39L signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CAB39L exon(s) critical for CAB39L function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CAB39L genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CAB39L CRISPR/Cas9 KO Plasmid (h) and CAB39L CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CAB39L locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CAB39L HDR Plasmid (h) and CAB39L HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CAB39L homology arms to support homology-directed repair at defined CAB39L target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.