Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CA VI CRISPR/Cas9 KO Plasmid (m): sc-419456
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CA VI CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CA VI genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CA VI Antibody (H-8): sc-514761
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CA VI CRISPR/Cas9 KO Plasmid (m) | sc-419456 | 20 µg | $397.00 |
Overview
Car6 encodes carbonic anhydrase VI (CA VI), a secreted zinc metalloenzyme that catalyzes the reversible hydration of CO₂ to bicarbonate and protons, supporting extracellular pH buffering and bicarbonate homeostasis in glandular and mucosal environments. By shaping proton dynamics, CA VI contributes to processes linked to epithelial ion transport and microenvironmental pH control that can influence enzyme activity, matrix interactions, and cellular signaling. In mouse systems, Car6 expression is often used to study carbonic anhydrase–dependent regulation of extracellular acid–base balance in secretory tissues. Dysregulated pH control is broadly relevant to inflammation, tissue remodeling, and metabolic stress models, making Car6 a useful target for mechanistic studies of pH-sensitive phenotypes.
CA VI CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Car6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Car6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Car6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CA VI protein expression.
This CRISPR knockout system enables efficient generation of Car6-deficient cell models for investigation of CA VI signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Car6 exon(s) critical for CA VI function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Car6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CA VI CRISPR/Cas9 KO Plasmid (m) and CA VI CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Car6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CA VI HDR Plasmid (m) and CA VI HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Car6 homology arms to support homology-directed repair at defined Car6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.