CA III Antibody (F-10): sc-373729

CA III Antibody (F-10) is a mouse monoclonal IgG3 kappa light chain antibody that detects CA III protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CA III (F-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Carbonic anhydrases (CAs) are a significant family of zinc metalloenzymes that play a crucial role in catalyzing the reversible hydration of carbon dioxide, which is vital for maintaining acid-base balance and facilitating various physiological processes. Specifically, CA III, also known as Car3 or CA3, is a 260 amino acid cytoplasmic protein predominantly expressed in muscle tissue, where CA III contributes to the regulation of pH and carbon dioxide levels during muscle metabolism. CA III′s unique structure, characterized by its dimeric form and zinc-binding site, is essential for enzymatic activity, allowing CA III to efficiently convert carbon dioxide to bicarbonate and protons. This function is particularly important in muscle cells, where rapid changes in pH can occur during intense physical activity. CA III is activated by proton donors such as imidazole and dipeptide histidylhistidine, while CA III activity can be inhibited by compounds like coumarins and sulfonamide derivatives, including acetazolamide, making CA III a target for therapeutic interventions in conditions related to acid-base imbalances.

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CA III Antibody (F-10) References:

1. Carbonic anhydrase inhibitors. Interaction of isozymes I, II, IV, V, and IX with phosphates, carbamoyl phosphate, and the phosphonate antiviral drug foscarnet.  |  Rusconi, S., et al. 2004. Bioorg Med Chem Lett. 14: 5763-7. PMID: 15501037
2. Carbonic anhydrase inhibitors. Inhibition of isozymes I, II, IV, V, and IX with anions isosteric and isoelectronic with sulfate, nitrate, and carbonate.  |  Innocenti, A., et al. 2005. Bioorg Med Chem Lett. 15: 567-71. PMID: 15664814
3. Carbonic anhydrase inhibitors. Interaction of isozymes I, II, IV, V, and IX with carboxylates.  |  Innocenti, A., et al. 2005. Bioorg Med Chem Lett. 15: 573-8. PMID: 15664815
4. Carbonic anhydrase inhibitors. Inhibition of isozymes I, II, IV, V and IX with complex fluorides, chlorides and cyanides.  |  Innocenti, A., et al. 2005. Bioorg Med Chem Lett. 15: 1909-13. PMID: 15780631
5. Comparison of the distribution of carbonic anhydrase isozymes (CA-I, CA-II, CA-III) in the rat gastrointestinal tract.  |  Igarashi, S., et al. 1992. J Vet Med Sci. 54: 535-9. PMID: 1643175
6. Mapping of mouse carbonic anhydrase-3, Car-3: another locus in the homologous region of mouse chromosome 3 and human chromosome 8.  |  Beechey, C., et al. 1990. Genomics. 6: 692-6. PMID: 2111277
7. The gene for human muscle specific carbonic anhydrase (CAIII) is assigned to chromosome 8.  |  Edwards, YH., et al. 1986. Ann Hum Genet. 50: 41-7. PMID: 3122635
8. Evaluation of carrier detection of Duchenne muscular dystrophy using carbonic anhydrase III and creatine kinase.  |  Heath, R., et al. 1985. Am J Med Genet. 21: 291-6. PMID: 3925781
9. Localisation and characterisation of carbonic anhydrase isozymes (CA I, CA II, CA III and CA IV) in an umbilical vein endothelial cell line (EA-hy926).  |  Mahieu, I., et al. 1995. Biochem Soc Trans. 23: 308S. PMID: 7672337

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CA III Antibody (F-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CA III Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CA III Antibody (F-10) Specifications

Alternate Product Name: Carbonic anhydrase 3 (CA3) muscle specific

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 27-55

Epitope Target: CA III

Epitope Species: human

Product Reactivity/Detection: CA III

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CA III Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin