CA III Antibody (E-4) recommended for detection of CA III of mouse, rat and human origin by WB, IP, IF and ELISA

CA III Antibody (E-4): sc-271345

CA III Antibody (E-4) is rated 5.0 out of 5 by 3.
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Datasheets

    • CA III Antibody (E-4) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 191-247 mapping near the C-terminus of CA III of human origin
    • recommended for detection of CA III of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What blocking solution should I use with CA III (E-4): sc-271345 monoclonal antibody for Western blot?

Asked by: TinTin
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Robust signal in WB in human skeletal muscle Robust signal in WB in human skeletal muscle tissue extract. -SCBT QC
Date published: 2015-01-30
Rated 5 out of 5 by from Positive signal in WB in Saos Positive signal in WB in Saos-2 whole cell lysate and mouse skeletal muscle tissue extract. -SCBT QC
Date published: 2015-01-15
Rated 5 out of 5 by from Strong band in WB in human transfected 293T Strong band in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-07-10
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