C5a Antibody (2942): sc-52633

C5a Antibody (2942) is a mouse monoclonal IgG2a antibody that detects C5a protein of human origin by western blotting (WB). Anti-C5a antibody (2942) is available as the non-conjugated monoclonal isotype antibody. C5a protein plays a crucial role in the immune response as a potent anaphylatoxin, which is released during the complement activation process. This activation is essential for the elimination of pathogens and involves a series of ligand-surface protein interactions specific to hematopoietic lineage cells. C5a precursor undergoes proteolytic cleavage to produce C5a, which interacts with G protein-coupled receptors on myeloid leukocytes, leading to a cascade of proinflammatory responses, including cellular degranulation, smooth muscle contraction, and cytokine release. C5a receptor (C5aR) is involved in signaling pathways that utilize the Ras-Raf-ERK1/2 cascade and is prevalent on various cell types, including hepatocytes and endothelial cells. C5a′s role in mediating inflammatory responses highlights its potential as a therapeutic target in conditions such as asthma and bacterial meningitis, where dysregulation can contribute to pathophysiological phenotypes.

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C5a Antibody (2942) References:

1. Modulation of C3a activity: internalization of the human C3a receptor and its inhibition by C5a.  |  Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
2. Ligand-induced phosphorylation of anaphylatoxin receptors C3aR and C5aR is mediated by 'G protein-coupled receptor kinases.  |  Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
3. A role for the C3a anaphylatoxin receptor in the effector phase of asthma.  |  Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
4. Role of C5a in inflammatory responses.  |  Guo, RF. and Ward, PA. 2005. Annu Rev Immunol. 23: 821-52. PMID: 15771587
5. Targeting complement component 5a promotes vascular integrity and limits airway remodeling.  |  Khan, MA., et al. 2013. Proc Natl Acad Sci U S A. 110: 6061-6. PMID: 23530212
6. C5a receptor (CD88) blockade protects against MPO-ANCA GN.  |  Xiao, H., et al. 2014. J Am Soc Nephrol. 25: 225-31. PMID: 24179165
7. Human complement component C3: cDNA coding sequence and derived primary structure.  |  de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
8. Intracellular complement C5a/C5aR1 stabilizes β-catenin to promote colorectal tumorigenesis.  |  Ding, P., et al. 2022. Cell Rep. 39: 110851. PMID: 35649359
9. C5a-licensed phagocytes drive sterilizing immunity during systemic fungal infection.  |  Desai, JV., et al. 2023. Cell. 186: 2802-2822.e22. PMID: 37220746
10. The role of C5a receptors in autoimmunity.  |  Schanzenbacher, J., et al. 2023. Immunobiology. 228: 152413. PMID: 37598588
11. Mitogen-activated protein kinase activation requires two signal inputs from the human anaphylatoxin C5a receptor.  |  Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
12. TNF-alpha-mediated expression of the receptor for anaphylatoxin C5a on neurons in experimental Listeria meningoencephalitis.  |  Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605

The preferred C5b-9 antibody is C5b-9 Antibody (aE11): sc-58935.