C4BPβ Antibody (E-1): sc-514553
- C4BPβ Antibody (E-1) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 82-252 mapping at the C-terminus of C4BPβ of human origin
- C4BP beta Antibody (E-1) is recommended for detection of C4BPβ of human origin by WB, IP, IF and ELISA
- Anti-C4BP beta Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for C4BPβ Antibody (E-1) for WB applications. These reagents are now offered in bundles with C4BPβ Antibody (E-1) (see ordering information below).
QUICK LINKS
SEE ALSO...
C4BPβ Antibody (E-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects C4BPβ of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-C4BPβ antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. C4BPβ plays a crucial role in regulating the complement system, particularly in the classical pathway of complement activation. C4BPβ maintains immune homeostasis by preventing excessive complement activation, which can lead to tissue damage and inflammation. C4BPβ is primarily located in the plasma and comprises multiple subunits, including α and β chains, forming a complex that binds to complement component C4, thereby accelerating C4′s inactivation. This interaction helps control active complement component levels in circulation, preventing unwanted immune responses. C4BPβ regulation of complement activation is particularly important in autoimmune diseases and inflammatory conditions, where dysregulation can lead to significant pathology. Using C4BPβ (E-1) monoclonal antibody enables researchers to gain insights into complement regulation mechanisms and their implications in various diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
C4BPβ Antibody (E-1) References:
- A cluster of positively charged amino acids in the C4BP alpha-chain is crucial for C4b binding and factor I cofactor function. | Blom, AM., et al. 1999. J Biol Chem. 274: 19237-45. PMID: 10383431
- Human C4b-binding protein has overlapping, but not identical, binding sites for C4b and streptococcal M proteins. | Blom, AM., et al. 2000. J Immunol. 164: 5328-36. PMID: 10799895
- Positively charged amino acids at the interface between alpha-chain CCP1 and CCP2 of C4BP are required for regulation of the classical C3-convertase. | Blom, AM., et al. 2000. Mol Immunol. 37: 445-53. PMID: 11090879
- Complement regulation in human atherosclerotic coronary lesions. Immunohistochemical evidence that C4b-binding protein negatively regulates the classical complement pathway, and that C5b-9 is formed via the alternative complement pathway. | Oksjoki, R., et al. 2007. Atherosclerosis. 192: 40-8. PMID: 16846604
- C4b-binding protein and factor H compensate for the loss of membrane-bound complement inhibitors to protect apoptotic cells against excessive complement attack. | Trouw, LA., et al. 2007. J Biol Chem. 282: 28540-28548. PMID: 17699521
- Molecular cloning and characterization of the cDNA coding for C4b-binding protein, a regulatory protein of the classical pathway of the human complement system. | Chung, LP., et al. 1985. Biochem J. 230: 133-41. PMID: 3840370
- Human C4-binding protein. I. Isolation and characterization. | Scharfstein, J., et al. 1978. J Exp Med. 148: 207-22. PMID: 670886
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C4BPβ Antibody (E-1) | sc-514553 | 200 µg/ml | $322.00 | |||
C4BPβ Antibody (E-1): m-IgGκ BP-HRP Bundle | sc-524765 | 200 µg Ab, 40 µg BP | $361.00 | |||
C4BPβ Antibody (E-1): m-IgG2b BP-HRP Bundle | sc-549182 | 200 µg Ab; 10 µg BP | $361.00 | |||
C4BPβ Antibody (E-1) AC | sc-514553 AC | 500 µg/ml, 25% agarose | $424.00 | |||
C4BPβ Antibody (E-1) HRP | sc-514553 HRP | 200 µg/ml | $322.00 | |||
C4BPβ Antibody (E-1) FITC | sc-514553 FITC | 200 µg/ml | $336.00 | |||
C4BPβ Antibody (E-1) PE | sc-514553 PE | 200 µg/ml | $349.00 | |||
C4BPβ Antibody (E-1) Alexa Fluor® 488 | sc-514553 AF488 | 200 µg/ml | $364.00 | |||
C4BPβ Antibody (E-1) Alexa Fluor® 546 | sc-514553 AF546 | 200 µg/ml | $364.00 | |||
C4BPβ Antibody (E-1) Alexa Fluor® 594 | sc-514553 AF594 | 200 µg/ml | $364.00 | |||
C4BPβ Antibody (E-1) Alexa Fluor® 647 | sc-514553 AF647 | 200 µg/ml | $364.00 | |||
C4BPβ Antibody (E-1) Alexa Fluor® 680 | sc-514553 AF680 | 200 µg/ml | $364.00 | |||
C4BPβ Antibody (E-1) Alexa Fluor® 790 | sc-514553 AF790 | 200 µg/ml | $364.00 |