C3G Antibody (G-9): sc-393836

C3G Antibody (G-9) is a mouse monoclonal IgG2b antibody that detects C3G in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). C3G plays a crucial role as a guanine nucleotide exchange factor (GEF) for Ras, a key regulator in various cellular processes, including cell growth, differentiation, and survival. C3G activates Ras by facilitating the exchange of GDP for GTP, which is vital for proper cellular signaling; dysregulation of this process can lead to oncogenic transformations and contribute to cancer progression. C3G is primarily located in the cytoplasm, where C3G interacts with other signaling molecules, including the Crk proto-oncogene product, to modulate Ras activity. This interaction highlights the intricate network of signaling pathways that govern cellular responses, and any alterations in these pathways can have profound implications for cell behavior and disease states. C3G gene is located on human chromosome 9q34.13, near the c-Abl gene, which is also implicated in oncogenic processes, further underscoring C3G′s importance in cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

C3G Antibody (G-9) References:

1. C3G overexpression promotes the survival of rat-derived H9C2 cardiomyocytes by p-ERK1/2.  |  Zhang, X., et al. 2013. Cell Biol Int. 37: 1106-13. PMID: 23686869
2. The C3G/Rap1 pathway promotes secretion of MMP-2 and MMP-9 and is involved in serous ovarian cancer metastasis.  |  Che, YL., et al. 2015. Cancer Lett. 359: 241-9. PMID: 25617801
3. C3G knock-down enhances migration and invasion by increasing Rap1-mediated p38α activation, while it impairs tumor growth through p38α-independent mechanisms.  |  Priego, N., et al. 2016. Oncotarget. 7: 45060-45078. PMID: 27286263
4. C3G dynamically associates with nuclear speckles and regulates mRNA splicing.  |  Shakyawar, DK., et al. 2018. Mol Biol Cell. 29: 1111-1124. PMID: 29496966
5. Silencing of C3G increases cardiomyocyte survival inhibition and apoptosis via regulation of p-ERK1/2 and Bax.  |  Yang, D., et al. 2019. Clin Exp Pharmacol Physiol. 46: 237-245. PMID: 30152875
6. C3G localizes to the mother centriole in a cenexin-dependent manner and regulates centrosome duplication and primary cilium length.  |  Nayak, SC. and Radha, V. 2020. J Cell Sci. 133: PMID: 32371504
7. Mechanisms of autoregulation of C3G, activator of the GTPase Rap1, and its catalytic deregulation in lymphomas.  |  Carabias, A., et al. 2020. Sci Signal. 13: PMID: 32873726
8. C3G Regulates STAT3, ERK, Adhesion Signaling, and Is Essential for Differentiation of Embryonic Stem Cells.  |  Vishnu, VV., et al. 2021. Stem Cell Rev Rep. 17: 1465-1477. PMID: 33624208
9. C3G downregulation induces the acquisition of a mesenchymal phenotype that enhances aggressiveness of glioblastoma cells.  |  Manzano, S., et al. 2021. Cell Death Dis. 12: 348. PMID: 33824275
10. The Interactional Characterization of Lentil Protein Isolate (LPI) with Cyanidin-3-O-Glucoside (C3G) and Their Effect on the Stability and Antioxidant Activity of C3G.  |  Qian, H., et al. 2022. Foods. 12: PMID: 36613320

The preferred C3G antibody is C3G Antibody (G-4): sc-17840.