C23 (Nucleolin) Antibody (MS-3): sc-8031

C23 Antibody (MS-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects C23 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). C23 monoclonal antibody (MS-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. C23, also known as nucleolin (NCL), is a crucial eukaryotic nucleolar phosphoprotein that plays a significant role in the synthesis and maturation of ribosomes, which are essential for protein synthesis in all living cells. C23 is primarily localized in the dense fibrillar regions of the nucleolus, where C23 contains four RNA binding domains that facilitate interaction with pre-ribosomal RNA during ribosome biogenesis. This localization ensures efficient processing of ribosomal RNA and assembly of ribosomal subunits, which are necessary for cellular function and growth. C23 interacts with various proteins, including nucleolar protein B23, and phosphorylation by Cdc2 and casein kinase II leads to translocation from the nucleolus to the cytoplasm, highlighting a dynamic role in cellular processes. C23 interaction with human telomerase reverse transcriptase subunit (TERT) is particularly important for nucleolar localization of TERT, further emphasizing significance in cellular regulation and stability.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

C23 (Nucleolin) Antibody (MS-3) References:

1. Nucleolin interacts with telomerase.  |  Khurts, S., et al. 2004. J Biol Chem. 279: 51508-15. PMID: 15371412
2. Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis.  |  Intine, RV., et al. 2004. Mol Cell Biol. 24: 10894-904. PMID: 15572691
3. Localization of phosphorylated forms of Bcl-2 in mitosis: co-localization with Ki-67 and nucleolin in nuclear structures and on mitotic chromosomes.  |  Barboule, N., et al. 2005. Cell Cycle. 4: 590-6. PMID: 15876860
4. Mitosis-specific phosphorylation of nucleolin by p34cdc2 protein kinase.  |  Belenguer, P., et al. 1990. Mol Cell Biol. 10: 3607-18. PMID: 2192260
5. C23 promotes tumorigenesis via suppressing p53 activity.  |  Li, Q., et al. 2016. Oncotarget. 7: 58274-58285. PMID: 27506938
6. Effects of anti-C23 (nucleolin) antibody on transcription of ribosomal DNA in Chironomus salivary gland cells.  |  Egyhazi, E., et al. 1988. Exp Cell Res. 178: 264-72. PMID: 3169130
7. Protein and cDNA sequence of a glycine-rich, dimethylarginine-containing region located near the carboxyl-terminal end of nucleolin (C23 and 100 kDa).  |  Lapeyre, B., et al. 1986. J Biol Chem. 261: 9167-73. PMID: 3755137
8. Localization of phosphoprotein C23 to nucleolar structures and to the nucleolus organizer regions.  |  Lischwe, MA., et al. 1981. Exp Cell Res. 136: 101-9. PMID: 7028492
9. C23 interacts with B23, a putative nucleolar-localization-signal-binding protein.  |  Li, YP., et al. 1996. Eur J Biochem. 237: 153-8. PMID: 8620867
10. Nucleolin functions in the first step of ribosomal RNA processing.  |  Ginisty, H., et al. 1998. EMBO J. 17: 1476-86. PMID: 9482744
11. A B-cell-specific DNA recombination complex.  |  Borggrefe, T., et al. 1998. J Biol Chem. 273: 17025-35. PMID: 9642267
12. Nucleolin interacts with several ribosomal proteins through its RGG domain.  |  Bouvet, P., et al. 1998. J Biol Chem. 273: 19025-9. PMID: 9668083

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-C23 (Nucleolin) Antibody (MS-3) has 327 citations in a variety of scientific publications.

Primary antibodies like Anti-C23 (Nucleolin) Antibody (MS-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

C23 (Nucleolin) Antibody (MS-3) Specifications

Alternate Product Name: Nucleolin

Product Clone Name: MS-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-706

Epitope Target: C23

Epitope Species: human

Product Reactivity/Detection: C23

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 327 citations in various scientific publications

Available Conjugates/Forms for C23 (Nucleolin) Antibody (MS-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine