Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
C1QBP CRISPR/Cas9 KO Plasmid (m): sc-419387
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C1QBP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C1QBP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: C1QBP Antibody (74.5.2): sc-23885
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C1QBP CRISPR/Cas9 KO Plasmid (m) | sc-419387 | 20 µg | $397.00 |
Overview
C1qbp encodes C1QBP (also known as gC1qR/p32), a multifunctional protein enriched in mitochondria that supports oxidative phosphorylation, mitochondrial ribosome function, and cellular energy metabolism. It also participates in RNA processing and regulates apoptosis and innate immune signaling through interactions with complement-related and pattern-recognition pathways. In mouse systems, altered C1QBP activity has been linked to mitochondrial stress responses, changes in inflammatory signaling, and susceptibility to metabolic and neurodegeneration-relevant phenotypes. These functions make C1QBP a useful node for dissecting mitochondria–immunity crosstalk and bioenergetic regulation in disease models.
C1QBP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the C1qbp gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the C1qbp together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the C1qbp open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C1QBP protein expression.
This CRISPR knockout system enables efficient generation of C1qbp-deficient cell models for investigation of C1QBP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting C1qbp exon(s) critical for C1QBP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple C1qbp genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C1QBP CRISPR/Cas9 KO Plasmid (m) and C1QBP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the C1qbp locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C1QBP HDR Plasmid (m) and C1QBP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by C1qbp homology arms to support homology-directed repair at defined C1qbp target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.