Src Antibody (H-12): sc-5266

c-Src Antibody (H-12) is a mouse monoclonal IgG2a kappa light chain antibody raised against amino acids 1-30 at the N-terminus of human c-Src, a non-receptor tyrosine kinase crucial for cellular signal transduction. c-Src mouse monoclonal antibody (H-12) recognizes an epitope within the N-terminal region and demonstrates broad species cross-reactivity, detecting c-Src in mouse, rat, human, and avian samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. As a member of the Src family kinases, c-Src regulates fundamental cellular processes including proliferation, differentiation, motility, and adhesion. c-Src′s unique subcellular distribution stems from N-terminal myristoylation, which anchors c-Src to the inner surface of the plasma membrane. This specific localization proves essential for c-Src′s function, positioning c-Src to interact with various cell surface receptors, including integrins and growth factor receptors, thereby enabling efficient transmission of extracellular signals into intracellular responses. When c-Src′s localization or activity becomes dysregulated, the resulting aberrant signaling can promote oncogenic transformation, drive cancer progression, and facilitate metastasis. Through these diverse cellular roles, c-Src serves as a critical mediator of cellular communication and response. Anti-c-Src monoclonal antibody (H-12) provides researchers with a valuable tool for investigating c-Src′s functions in both normal physiology and disease conditions, particularly in understanding signal transduction pathways and developing targeted therapeutic strategies.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Src Antibody (H-12) has 103 citations in a variety of scientific publications.

Primary antibodies like Anti-Src Antibody (H-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Src Antibody (H-12) Specifications

Alternate Product Name: SRC

Product Clone Name: H-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-30

Epitope Target: c-Src

Epitope Species: human

Product Reactivity/Detection: c-Src

Species Reactivity/Detection: mouse, rat, human, avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 103 citations in various scientific publications

Available Conjugates/Forms for Src Antibody (H-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Src antibody is Src Antibody (B-12): sc-8056.