C-Nap1 Antibody (F-7): sc-390540
- C-Nap1 Antibody (F-7) is a mouse monoclonal IgG2a κ C-Nap1 antibody, cited in 15 publications, provided at 200 µg/ml
- raised against amino acids 107-286 mapping near the N-terminus of C-Nap1 of human origin
- C-Nap1 Antibody (F-7) is recommended for detection of C-Nap1 of human origin by WB, IP, IF and ELISA
- Anti-C-Nap1 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of C-Nap1 (F-7): sc-390540.
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for C-Nap1 Antibody (F-7) for WB applications. These reagents are now offered in bundles with C-Nap1 Antibody (F-7) (see ordering information below).
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SEE ALSO...
C-Nap1 Antibody (F-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects C-Nap1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-C-Nap1 antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. C-Nap1, also known as Centrosomal Nek2-associated protein 1 or CEP2, is a centrosome-associated protein that plays a crucial role in organizing and functioning of centrioles, which are essential for proper cell division and cilia formation. C-Nap1 localizes to the cytoplasm in all human cells, existing as three isoforms and comprising 2,442 amino acids. C-Nap1 facilitates centriole cohesion during interphase, ensuring proper timing for cell division. Phosphorylation of C-Nap1, especially by Nek2 during mitosis, regulates centrosome dissociation, a process critical for accurate chromosome segregation. Reaccumulation of C-Nap1 in centrosomes after cellular division highlights a dynamic role in cell cycle regulation. C-Nap1 antibodies present in certain autoimmune diseases suggest involvement in disease pathogenesis, making C-Nap1 a significant target for research in both cell biology and immunology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
C-Nap1 Antibody (F-7) References:
- The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion. | Mayor, T., et al. 2000. J Cell Biol. 151: 837-46. PMID: 11076968
- The mechanism regulating the dissociation of the centrosomal protein C-Nap1 from mitotic spindle poles. | Mayor, T., et al. 2002. J Cell Sci. 115: 3275-84. PMID: 12140259
- Rootletin interacts with C-Nap1 and may function as a physical linker between the pair of centrioles/basal bodies in cells. | Yang, J., et al. 2006. Mol Biol Cell. 17: 1033-40. PMID: 16339073
- Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion. | Graser, S., et al. 2007. J Cell Sci. 120: 4321-31. PMID: 18042621
- beta-Catenin is a Nek2 substrate involved in centrosome separation. | Bahmanyar, S., et al. 2008. Genes Dev. 22: 91-105. PMID: 18086858
- A novel function of CEP135 as a platform protein of C-NAP1 for its centriolar localization. | Kim, K., et al. 2008. Exp Cell Res. 314: 3692-700. PMID: 18851962
- Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion. | Fang, G., et al. 2014. J Cell Sci. 127: 1631-9. PMID: 24554434
- Multisite phosphorylation of C-Nap1 releases it from Cep135 to trigger centrosome disjunction. | Hardy, T., et al. 2014. J Cell Sci. 127: 2493-506. PMID: 24695856
- The tumor suppressor proteins ASPP1 and ASPP2 interact with C-Nap1 and regulate centrosome linker reassembly. | Zhang, Y., et al. 2015. Biochem Biophys Res Commun. 458: 494-500. PMID: 25660448
- Centrosome linker protein C-Nap1 maintains stem cells in mouse testes. | Dang, H., et al. 2022. EMBO Rep. 23: e53805. PMID: 35599622
- C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. | Fry, AM., et al. 1998. J Cell Biol. 141: 1563-74. PMID: 9647649
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C-Nap1 Antibody (F-7) | sc-390540 | 200 µg/ml | $316.00 | |||
C-Nap1 Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-530182 | 200 µg Ab; 10 µg BP | $354.00 | |||
C-Nap1 Antibody (F-7): m-IgGκ BP-HRP Bundle | sc-523575 | 200 µg Ab, 40 µg BP | $354.00 | |||
C-Nap1 Antibody (F-7) AC | sc-390540 AC | 500 µg/ml, 25% agarose | $416.00 | |||
C-Nap1 Antibody (F-7) HRP | sc-390540 HRP | 200 µg/ml | $316.00 | |||
C-Nap1 Antibody (F-7) FITC | sc-390540 FITC | 200 µg/ml | $330.00 | |||
C-Nap1 Antibody (F-7) PE | sc-390540 PE | 200 µg/ml | $343.00 | |||
C-Nap1 Antibody (F-7) Alexa Fluor® 488 | sc-390540 AF488 | 200 µg/ml | $357.00 | |||
C-Nap1 Antibody (F-7) Alexa Fluor® 546 | sc-390540 AF546 | 200 µg/ml | $357.00 | |||
C-Nap1 Antibody (F-7) Alexa Fluor® 594 | sc-390540 AF594 | 200 µg/ml | $357.00 | |||
C-Nap1 Antibody (F-7) Alexa Fluor® 647 | sc-390540 AF647 | 200 µg/ml | $357.00 | |||
C-Nap1 Antibody (F-7) Alexa Fluor® 680 | sc-390540 AF680 | 200 µg/ml | $357.00 | |||
C-Nap1 Antibody (F-7) Alexa Fluor® 790 | sc-390540 AF790 | 200 µg/ml | $357.00 |