c-Myb Antibody (D-7): sc-74512

c-Myb Antibody (D-7) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect c-Myb protein, also known as Myeloblastosis viral oncogene homolog c-Myb, MYB proto-oncogene, or c-Myb transcription factor. Raised against amino acids 500-640 of human c-Myb, c-Myb monoclonal antibody (D-7) effectively recognizes c-Myb proteins from mouse, rat, and human species in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). c-Myb protein is predominantly localized in the nucleus, where c-Myb functions as a critical transcription factor regulating the expression of genes involved in hematopoietic cell proliferation, differentiation, and survival. This nuclear localization is essential for c-Myb′s role in controlling transcriptional programs that maintain the balance between cell growth and differentiation, making c-Myb a vital component in normal hematopoiesis and implicated in oncogenic processes when dysregulated. anti-c-Myb antibody (D-7) is available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP), fluorescein isothiocyanate (FITC), phycoerythrin (PE), and Alexa Fluor® conjugates, providing flexibility for diverse experimental setups. By facilitating the study of c-Myb′s nuclear functions, c-Myb (D-7) antibody supports advanced research into molecular mechanisms underlying blood cell development and related diseases. Whether investigating normal hematopoietic processes or exploring pathways involved in leukemogenesis, c-Myb monoclonal antibody (D-7) is an indispensable tool for researchers aiming to elucidate critical roles of the Myb family of transcription factors.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

c-Myb Antibody (D-7) References:

1. The chromatin remodeling factor Mi-2alpha acts as a novel co-activator for human c-Myb.  |  Saether, T., et al. 2007. J Biol Chem. 282: 13994-4005. PMID: 17344210
2. c-Myb is required for progenitor cell homeostasis in colonic crypts.  |  Malaterre, J., et al. 2007. Proc Natl Acad Sci U S A. 104: 3829-34. PMID: 17360438
3. Increase in specific DNA binding by carboxyl truncation suggests a mechanism for activation of Myb.  |  Ramsay, RG., et al. 1991. Oncogene. 6: 1875-9. PMID: 1923510
4. DNA binding activity and transcriptional activator function of the human B-myb protein compared with c-MYB.  |  Mizuguchi, G., et al. 1990. J Biol Chem. 265: 9280-4. PMID: 2160970
5. c-Myb Binding Sites in Haematopoietic Chromatin Landscapes.  |  Bengtsen, M., et al. 2015. PLoS One. 10: e0133280. PMID: 26208222
6. Delineation of three functional domains of the transcriptional activator encoded by the c-myb protooncogene.  |  Sakura, H., et al. 1989. Proc Natl Acad Sci U S A. 86: 5758-62. PMID: 2668947
7. Nucleotide sequence of cDNA clones of the murine myb proto-oncogene.  |  Gonda, TJ., et al. 1985. EMBO J. 4: 2003-8. PMID: 2998780
8. The transcription factor c-Myb regulates CD8+ T cell stemness and antitumor immunity.  |  Gautam, S., et al. 2019. Nat Immunol. 20: 337-349. PMID: 30778251
9. c-Myb regulates tumorigenic potential of embryonal rhabdomyosarcoma cells.  |  Kaspar, P., et al. 2019. Sci Rep. 9: 6342. PMID: 31004084
10. Expression of myb, myc and fos proto-oncogenes during the differentiation of a murine myeloid leukaemia.  |  Gonda, TJ. and Metcalf, D. Nature. 310: 249-51. PMID: 6205276
11. Constitutive expression of B-myb can bypass p53-induced Waf1/Cip1-mediated G1 arrest.  |  Lin, D., et al. 1994. Proc Natl Acad Sci U S A. 91: 10079-83. PMID: 7937841
12. Detection of proteins that bind to the leucine zipper motif of c-Myb.  |  Favier, D. and Gonda, TJ. 1994. Oncogene. 9: 305-11. PMID: 8302594

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-c-Myb Antibody (D-7) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-c-Myb Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

c-Myb Antibody (D-7) Specifications

Alternate Product Name: c-Myb transcription factor

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 500-640

Epitope Target: c-Myb

Epitope Species: human

Product Reactivity/Detection: c-Myb

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for c-Myb Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent