c-GMP Antibody (8D8): sc-21727
- c-GMP Antibody (8D8) is a mouse monoclonal IgG2b κ c-GMP antibody, cited in 3 publications, provided at 200 µg/ml
- raised against integral membrane protein from rat liver fraction enriched in Golgi cisternae
- c-GMP Antibody (8D8) is recommended for detection of c-GMP of mouse and rat origin by WB, IP and IF
- Anti-c-GMP Antibody (8D8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for c-GMP Antibody (8D8) for WB applications. This reagent is now offered in a bundle with c-GMP Antibody (8D8) (see ordering information below).
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c-GMP Antibody (8D8) is a mouse monoclonal IgG2b kappa light chain antibody that detects c-GMP protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-c-GMP antibody (8D8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. c-GMP, also known as cis-Golgi membrane protein, plays a crucial role in the organization and function of the Golgi apparatus, which is essential for the post-translational modification and sorting of proteins synthesized in the endoplasmic reticulum. c-GMP is localized to the cis and medial cisternae of the Golgi stack, where c-GMP participates in the processing of proteins through various modifications such as glycosylation and phosphorylation. These modifications are vital for the proper functioning of proteins, influencing their stability, activity, and interactions with other cellular components. The Golgi apparatus itself is structured with distinct compartments that house different enzymes, allowing for a sequential modification process that is critical for the maturation of proteins before they are transported to their final destinations. Understanding c-GMP′s role in this context is important for elucidating the mechanisms of protein trafficking and processing within the cell, which has implications for various biological processes and diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
c-GMP Antibody (8D8) References:
- In vitro generation from the trans-Golgi network of coatomer-coated vesicles containing sialylated vesicular stomatitis virus-G protein. | Simon, JP., et al. 2000. Methods. 20: 437-54. PMID: 10720465
- Assembly and disassembly of the Golgi complex: two processes arranged in a cis-trans direction. | Alcalde, J., et al. 1992. J Cell Biol. 116: 69-83. PMID: 1730750
- Two integral membrane proteins located in the cis-middle and trans-part of the Golgi system acquire sialylated N-linked carbohydrates and display different turnovers and sensitivity to cAMP-dependent phosphorylation. | Yuan, L., et al. 1987. J Cell Biol. 105: 215-27. PMID: 3301866
- Biosynthesis, glycosylation, movement through the Golgi system, and transport to lysosomes by an N-linked carbohydrate-independent mechanism of three lysosomal integral membrane proteins. | Barriocanal, JG., et al. 1986. J Biol Chem. 261: 16755-63. PMID: 3782140
- Two Golgi integral membrane proteins (GIMPS) exhibit region- and cell type-specific distribution in the epididymis of the adult rat. | Suarez-Quian, CA. and Jelesoff, N. 1994. Microsc Res Tech. 29: 481-91. PMID: 7873795
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
c-GMP Antibody (8D8) | sc-21727 | 200 µg/ml | $316.00 | |||
c-GMP Antibody (8D8): m-IgG Fc BP-HRP Bundle | sc-525502 | 200 µg Ab; 10 µg BP | $354.00 | |||
c-GMP Antibody (8D8) AC | sc-21727 AC | 500 µg/ml, 25% agarose | $416.00 | |||
c-GMP Antibody (8D8) HRP | sc-21727 HRP | 200 µg/ml | $316.00 | |||
c-GMP Antibody (8D8) FITC | sc-21727 FITC | 200 µg/ml | $330.00 | |||
c-GMP Antibody (8D8) PE | sc-21727 PE | 200 µg/ml | $343.00 | |||
c-GMP Antibody (8D8) Alexa Fluor® 488 | sc-21727 AF488 | 200 µg/ml | $357.00 | |||
c-GMP Antibody (8D8) Alexa Fluor® 546 | sc-21727 AF546 | 200 µg/ml | $357.00 | |||
c-GMP Antibody (8D8) Alexa Fluor® 594 | sc-21727 AF594 | 200 µg/ml | $357.00 | |||
c-GMP Antibody (8D8) Alexa Fluor® 647 | sc-21727 AF647 | 200 µg/ml | $357.00 | |||
c-GMP Antibody (8D8) Alexa Fluor® 680 | sc-21727 AF680 | 200 µg/ml | $357.00 | |||
c-GMP Antibody (8D8) Alexa Fluor® 790 | sc-21727 AF790 | 200 µg/ml | $357.00 |