Anti-Fos/c-Fos Antibody (E-8) recommended for detection of c-Fos, Fos B, Fra-1 and Fra-2 of mouse, rat, human, zebrafish and origin by WB, IP, IF, IHC(P) and ELISA

Anti-Fos/c-Fos Antibody (E-8): sc-166940

Anti-Fos/c-Fos Antibody (E-8) is rated 4.1 out of 5 by 44.
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Datasheets

    • Anti-Fos/c-Fos Antibody (E-8) is a mouse monoclonal IgG1 κ Fos/c-Fos antibody, cited in 125 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 128-152 within an internal region of c-Fos of human origin
    • Fos/c-Fos Antibody (E-8) is recommended for detection of c-Fos, Fos B, Fra-1 and Fra-2 of mouse, rat, human, zebrafish and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-Fos/c-Fos Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-166940 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Fos/c-Fos (E-8): sc-166940.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Fos/c-Fos Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Fos/c-Fos Antibody (E-8) (see ordering information below).
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sc-166940这只c-fos的抗体,我染小鼠脑组织的冰冻切片,0.3%Triton打孔10分钟,山羊血清室温封闭一小时,一抗4℃过夜,使用Alexa Fluo 488的二抗室温避光孵育1小时,但无阳性信号出现,请问问题出在哪里了呢?

Asked by: pangpangmao
感谢您的提问。我们很遗憾地获悉,这种抗体没有达到您的预期。我们希望你的研究取得成功。请联系我们的技术支持部门,亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。 Thank you for your question. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody.
Answered by: SCBT Representative
Date published: 2022-07-07

What temperature is suitable for storing and reusing c-fos antibody (Anti-Fos/c-Fos Antibody (E-8) X) following dilution, 4C, or -20C freezer?

Asked by: Parisa
We do not recommend freezing our antibodies. Recommended storage is 4C.
Answered by: Anonymous
Date published: 2022-04-07

I have a question about Anti-Fos/c-Fos Antibody (E-8) X (catalog: sc-166940 X) for western blot analysis. I would like to know if it is possible to use both HRP and fluorescent dye conjugated secondary antibodies for detecting the sc-166940 X antibody.

Asked by: Parisa
Thank you for your question. This primary antibody can be detected by either fluorescence-, or by an HRP-based secondary detection. Our secondary binding proteins m-IgG1 BP-HRP: sc-525408 and m-IgG1 BP-CFL 488: sc-533661 have been optimised for detecting primary mouse IgG1 antibodies including this one.
Answered by: Tech Support Europe
Date published: 2022-02-17

Hello, The c-Fos Antibody (K-25): sc-253 was discontinued and replaced by c-Fos Antibody (E-8): sc-166940? In what way are they the same? sc-253 has been shown to work for fish c-fos detection, so will sc-166940 work in the same way? Cheers!

Asked by: Yatz2020
Thank you for your question. c-Fos Antibody (E-8): sc-166940 is a mouse monoclonal IgG1 antibody that was raised against the same peptide antigen that was used to develop the rabbit polyclonal antibody, c-Fos Antibody (K-25): sc-253. The mouse monoclonal antibody has also been published in zebrafish samples, and is recommended to work for zebrafish. For some examples of literature using this mouse monoclonal antibody in zebrafish, please refer on our website to the product citations with PMID's: 30222997, 29626809 and 29061647.
Answered by: Technical Support
Date published: 2020-03-12

Is the c-Fos Antibody (E-8): sc-166940 tested for flow cytometry or if you could suggest any published references that have used this antibody for flow cytometry? Thanks!!

Asked by: Hema
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-08-20

I would like to determine the c-Fos expression in the mouse whole brain cleared with CUBIC or iDISCO. Have you tried to use E-8 for that?

Asked by: Atsu
Thank you for your question. We have not tested this antibody in whole brain tissues, or using either CUBIC or iDISCO.
Answered by: Technical Support
Date published: 2019-07-30

Good afternoon, We have found that this antibody must be used at a very high concentration in order to have any signal that is beyond the threshold of noise. Is this typical for this antibody or lot specific?

Asked by: Jordan Hopkins
Thank you for your question. We routinely use this antibody at the starting dilution of 1:100 (range 1:100-1:1,000) for Western blotting.
Answered by: Technical Support
Date published: 2019-07-10

Hi, I'm doing WB with c-Fos antibody (E-8) sc-166940, Datasheets shows that the Molecular Weight of c-Fos is 62 kDa, but in the images the WB they present is around 40 kDa, I could say the molecular weight that this antibody detects?

Asked by: Mar400
Thank you for your question. The predicted molecular weight of c-Fos is ~41 kDa. However, the protein is also subject to various post-translational modifications which may lead to a higher observed molecular weight. This antibody can also detect Fos B, which has an expected molecular weight of 45 kDa. The current molecular weight on our data sheet of 62 kDa is based on the following publication which describes serum-stimulated cells then immunoprecipitated for c-Fos, PMID: 2110368.
Answered by: Technical Support
Date published: 2019-05-25
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Rated 1 out of 5 by from Does not work for ChIP in mouse neurons I tested this antibody for ChIP in mouse primary neurons after stimulation, and there was no detectable enrichment by qPCR. I had used the same protocol for Egr1 ChIP (a similar inducible transcription factor) and it works nicely. Also, quite some background in WB.
Date published: 2022-03-09
Rated 5 out of 5 by from It can be used It can be used for osteoclasts which obtained from mouse.
Date published: 2021-07-21
Rated 5 out of 5 by from 条带清晰,效价高 之前用的A牌的抗体,就是不出,然后就申请了santa cruz的,想试试,没想到出图效果特别好,条带清晰,效价高
Date published: 2021-07-09
Rated 1 out of 5 by from Per immunofluorescenza non funziona! Ho provato questo anticorpo in immunofluorescenza su spinal cord di topo criostatato (diluizione 1:100), purtroppo senza alcun risultato positivo. Proverò ad utilizzarlo anche per wester blot
Date published: 2020-11-03
Rated 5 out of 5 by from Amazing in CLARITY I use this antibody in CLARITY and it has consistently produced the best images I have ever taken.
Date published: 2020-03-10
Rated 5 out of 5 by from c-Fos antibody I ordered this antibody last week. I think this antibody is very good.
Date published: 2018-05-08
Rated 2 out of 5 by from It does not work well I tested it by 1:500 dilutions compared with other company's ab, this one did not work well.
Date published: 2018-04-03
Rated 3 out of 5 by from Not a good alternative to sc-52 Not a very good alternative to sc-52, but comparable staining present at 1:50.
Date published: 2018-03-14
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