c-Fos Antibody (E-8): sc-166940

c-Fos Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 128-152 of human c-Fos, a nuclear transcription factor essential for gene regulation. Anti-c-Fos antibody (E-8) recognizes an epitope within this region and demonstrates remarkably broad species cross-reactivity, detecting c-Fos, FosB, Fra-1, and Fra-2 in mouse, rat, human, zebrafish, equine, canine, bovine, porcine, and avian samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. While c-Fos primarily operates in the nucleus as a component of the AP-1 complex to regulate genes controlling cell proliferation, differentiation, and survival, c-Fos′s distribution patterns show interesting complexity. For instance, in pancreatic cancer cells, c-Fos appears in both nuclear and cytoplasmic locations, suggesting broader regulatory roles than previously understood. This dynamic localization becomes particularly significant when c-Fos regulation goes awry - overexpression or dysregulation of c-Fos can contribute to cancer development, while c-Fos also plays crucial roles in mediating apoptosis and cellular stress responses. Through these diverse cellular functions and broad species applications, c-Fos (E-8) antibody provides researchers with a valuable tool for investigating transcriptional regulation, stress responses, and cancer biology across multiple model systems.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-c-Fos Antibody (E-8) has 171 citations in a variety of scientific publications.

Primary antibodies like Anti-c-Fos Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

c-Fos Antibody (E-8) Specifications

Alternate Product Name: c-Fos

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 128-152

Epitope Target: c-Fos

Epitope Species: human

Product Reactivity/Detection: c-Fos, Fos B, Fra-1 and Fra-2

Species Reactivity/Detection: mouse, rat, human, zebrafish

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 171 citations in various scientific publications

Available Conjugates/Forms for c-Fos Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred c-Fos antibody is c-Fos Antibody (D-1): sc-8047.