BTBD2 Antibody (H-2): sc-393927
- BTBD2 Antibody (H-2) is a mouse monoclonal IgG1 κ BTBD2 antibody provided at 200 µg/ml
- raised against amino acids 1-43 mapping at the N-terminus of BTBD2 of human origin
- BTBD2 Antibody (H-2) is recommended for detection of BTBD2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-BTBD2 Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for BTBD2 Antibody (H-2) for WB applications. These reagents are now offered in bundles with BTBD2 Antibody (H-2) (see ordering information below).
BTBD2 Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects BTBD2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BTBD2 (H-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BTBD2 protein, also known as BTB/POZ domain-containing protein 2, consists of 525 amino acids and features a single BTB/POZ domain, which is crucial for mediating both homomeric and heteromeric interactions among proteins that contain this domain. This structural characteristic enables BTBD2 to participate in forming transcriptional regulatory complexes, particularly those involved in chromatin remodeling. The BTB/POZ domain interacts with the SMRT/N-CoR-mSin3A histone deacetylase complex, playing a vital role in repressing and silencing gene transcription. Deletion of the BTB/POZ domain can lead to transcriptional repression loss, as seen in the oncogenic PLZF-RAR chimera associated with promyelocytic leukemias. BTBD2 is expressed in two isoforms due to alternative splicing and interacts with other proteins such as Topoisomerase I and Trim5δ, highlighting potential involvement in various cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BTBD2 Antibody (H-2) References:
- Recruitment of SMRT/N-CoR-mSin3A-HDAC-repressing complexes is not a general mechanism for BTB/POZ transcriptional repressors: the case of HIC-1 and gammaFBP-B. | Deltour, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 14831-6. PMID: 10611298
- Identification and characterization of BTBD1, a novel BTB domain containing gene on human chromosome 15q24. | Carim-Todd, L., et al. 2001. Gene. 262: 275-81. PMID: 11179693
- Characterization of BTBD1 and BTBD2, two similar BTB-domain-containing Kelch-like proteins that interact with Topoisomerase I. | Xu, L., et al. 2002. BMC Genomics. 3: 1. PMID: 11818025
- Gene and peptide analyses of newly defined lung cancer antigens recognized by HLA-A2402-restricted tumor-specific cytotoxic T lymphocytes. | Yamada, A., et al. 2003. Cancer Res. 63: 2829-35. PMID: 12782588
- BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta. | Xu, L., et al. 2003. Exp Cell Res. 288: 84-93. PMID: 12878161
- Search for cellular partners of human papillomavirus type 16 E2 protein. | Olejnik-Schmidt, AK., et al. 2008. Arch Virol. 153: 983-90. PMID: 18305892
- Components of the SMRT corepressor complex exhibit distinctive interactions with the POZ domain oncoproteins PLZF, PLZF-RARalpha, and BCL-6. | Wong, CW. and Privalsky, ML. 1998. J Biol Chem. 273: 27695-702. PMID: 9765306
- Crystal structure of the BTB domain from PLZF. | Ahmad, KF., et al. 1998. Proc Natl Acad Sci U S A. 95: 12123-8. PMID: 9770450
- The BCL-6 POZ domain and other POZ domains interact with the co-repressors N-CoR and SMRT. | Huynh, KD. and Bardwell, VJ. 1998. Oncogene. 17: 2473-84. PMID: 9824158
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BTBD2 Antibody (H-2) | sc-393927 | 200 µg/ml | $322.00 | |||
BTBD2 Antibody (H-2): m-IgG Fc BP-HRP Bundle | sc-527718 | 200 µg Ab; 10 µg BP | $361.00 | |||
BTBD2 Antibody (H-2): m-IgG1 BP-HRP Bundle | sc-533091 | 200 µg Ab; 20 µg BP | $361.00 | |||
BTBD2 Antibody (H-2) AC | sc-393927 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BTBD2 Antibody (H-2) HRP | sc-393927 HRP | 200 µg/ml | $322.00 | |||
BTBD2 Antibody (H-2) FITC | sc-393927 FITC | 200 µg/ml | $336.00 | |||
BTBD2 Antibody (H-2) PE | sc-393927 PE | 200 µg/ml | $349.00 | |||
BTBD2 Antibody (H-2) Alexa Fluor® 488 | sc-393927 AF488 | 200 µg/ml | $364.00 | |||
BTBD2 Antibody (H-2) Alexa Fluor® 546 | sc-393927 AF546 | 200 µg/ml | $364.00 | |||
BTBD2 Antibody (H-2) Alexa Fluor® 594 | sc-393927 AF594 | 200 µg/ml | $364.00 | |||
BTBD2 Antibody (H-2) Alexa Fluor® 647 | sc-393927 AF647 | 200 µg/ml | $364.00 | |||
BTBD2 Antibody (H-2) Alexa Fluor® 680 | sc-393927 AF680 | 200 µg/ml | $364.00 | |||
BTBD2 Antibody (H-2) Alexa Fluor® 790 | sc-393927 AF790 | 200 µg/ml | $364.00 |