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BSA Antibody (2A3E6): sc-32816

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Datasheets
  • BSA Antibody (2A3E6) is a mouse monoclonal IgG1 κ BSA antibody, cited in 2 publications, provided at 200 µg/ml
  • raised against bovine serum albumin
  • BSA Antibody (2A3E6) is recommended for detection of BSA of bovine origin by WB, IP and IF; non cross-reactive with human, rabbit, canine or guinea pig albumins
  • Anti-BSA Antibody (2A3E6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BSA Antibody (2A3E6) for WB applications. These reagents are now offered in bundles with BSA Antibody (2A3E6) (see ordering information below).
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BSA Antibody (2A3E6) is a mouse monoclonal IgG1 kappa light chain antibody that detects BSA protein of bovine origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). BSA (2A3E6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bovine serum albumin (BSA) plays a crucial role as the most abundant plasma protein in cows, essential for maintaining osmotic pressure in blood plasma, which ensures proper distribution of body fluids between intravascular compartments and body tissues. BSA′s structure consists of a single polypeptide chain that folds into a heart-shaped conformation, allowing BSA to bind various ligands, including fatty acids, hormones, and drugs. This unique structure contributes to BSA′s solubility and stability while facilitating function as a carrier protein, making BSA an invaluable reagent in laboratories for developing immunoassays. BSA′s numerous functional groups, particularly lysine residues, enable effective conjugation with other molecules, enhancing utility in various biochemical applications.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

BSA Antibody (2A3E6) References:

  1. Effect of bovine serum albumin (BSA) on enzymatic cellulose hydrolysis.  |  Wang, H., et al. 2013. Appl Biochem Biotechnol. 170: 541-51. PMID: 23553108
  2. Residual bovine serum albumin (BSA) quantitation in vaccines using automated Capillary Western technology.  |  Loughney, JW., et al. 2014. Anal Biochem. 461: 49-56. PMID: 24841366
  3. Bovine Serum Albumin (BSA) coated iron oxide magnetic nanoparticles as biocompatible carriers for curcumin-anticancer drug.  |  Nosrati, H., et al. 2018. Bioorg Chem. 76: 501-509. PMID: 29310081
  4. Poly(allylamine hydrochloride) (PAH) and Bovine Serum Albumin (BSA) Protein Nanostructured as Layer-by-Layer Thin Films.  |  Sa, AWL., et al. 2018. J Nanosci Nanotechnol. 18: 3908-3915. PMID: 29442726
  5. EGFR Targeted Cetuximab-Valine-Citrulline (vc)-Doxorubicin Immunoconjugates- Loaded Bovine Serum Albumin (BSA) Nanoparticles for Colorectal Tumor Therapy.  |  Ye, Z., et al. 2021. Int J Nanomedicine. 16: 2443-2459. PMID: 33814909
  6. Interaction of a bovine serum albumin (BSA) protein with mixed anionic-cationic surfactants and the resultant structure.  |  Saha, D., et al. 2021. Soft Matter. 17: 6972-6984. PMID: 34236073
  7. Interaction among bovine serum albumin (BSA) molecules in the presence of anions: a small-angle neutron scattering study.  |  Pandit, S., et al. 2022. J Biol Phys. 48: 237-251. PMID: 35416637
  8. Assessment on binding characteristics of ethiprole and a model protein bovine serum albumin (BSA) through various spectroscopic techniques integrated with computer simulation.  |  Jiang, SL., et al. 2023. J Biomol Struct Dyn. 41: 7862-7873. PMID: 36152999
  9. Mussel inspired multifunctional bovine serum albumin (BSA) coatings loaded with Baicalein (BAI) to enhance osteogenesis and resist oxidative stress for potential application on implant.  |  Yang, S., et al. 2023. Int J Biol Macromol. 229: 752-765. PMID: 36586655
  10. Antibodies to bovine serum albumin (BSA) in type 1 diabetes and other autoimmune disorders.  |  Füchtenbusch, M., et al. 1997. Exp Clin Endocrinol Diabetes. 105: 86-91. PMID: 9137938

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

BSA Antibody (2A3E6)

sc-32816
200 µg/ml
$322.00

BSA Antibody (2A3E6): m-IgG Fc BP-HRP Bundle

sc-528476
200 µg Ab; 10 µg BP
$361.00

BSA Antibody (2A3E6): m-IgGκ BP-HRP Bundle

sc-520866
200 µg Ab, 40 µg BP
$361.00

BSA Antibody (2A3E6): m-IgG1 BP-HRP Bundle

sc-542954
200 µg Ab; 20 µg BP
$361.00

BSA Antibody (2A3E6) AC

sc-32816 AC
500 µg/ml, 25% agarose
$424.00

BSA Antibody (2A3E6) HRP

sc-32816 HRP
200 µg/ml
$322.00

BSA Antibody (2A3E6) FITC

sc-32816 FITC
200 µg/ml
$336.00

BSA Antibody (2A3E6) PE

sc-32816 PE
200 µg/ml
$349.00

BSA Antibody (2A3E6) Alexa Fluor® 488

sc-32816 AF488
200 µg/ml
$364.00

BSA Antibody (2A3E6) Alexa Fluor® 546

sc-32816 AF546
200 µg/ml
$364.00

BSA Antibody (2A3E6) Alexa Fluor® 594

sc-32816 AF594
200 µg/ml
$364.00

BSA Antibody (2A3E6) Alexa Fluor® 647

sc-32816 AF647
200 µg/ml
$364.00

BSA Antibody (2A3E6) Alexa Fluor® 680

sc-32816 AF680
200 µg/ml
$364.00

BSA Antibody (2A3E6) Alexa Fluor® 790

sc-32816 AF790
200 µg/ml
$364.00

for sc-32816 one table shows epitope as FL (bovine) but I can not seem to find out what FL indicates

Asked by: Darrell
Thank you for your question. In the epitope description on the antibody table, FL is an abbreviation for "full-length", indicating the antibody was raised against an antigen sequence that is the full length protein, rather than a small peptide representing a specific region (e.g., N-terminus or C-terminus). In the case of this specific antibody, it was raised against bovine serum albumin, and not a recombinant or synthetic protein sequence. The exact epitope recognized by this monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-08-01

Are there any references for using this antibody in IHC(P)?

Asked by: hpatel
BSA Antibody (2A3E6): sc-32816 is only recommended for detection of BSA of bovine origin by WB, IP and IF. We do not recommend this antibody for IHC(P) applications. There currently is no published data indicating that the antibody would also be suitable for IHC(P).
Answered by: Tech Support Europe
Date published: 2019-07-17

What is the recommended fixation method for immunofluorescence staining with BSA (2A3E6): sc-32816 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Positive results in Western Blot analysisWe have tested this antibody in Western Blot against pure BSA samples. It worked really well with strong and clear signals around 65 kDa (dilution 1:1000 in PBS 0.1% non-fat-dried milk and 0.05% Tween20; 2h incubation at room temperature).
Date published: 2022-04-12
Rated 5 out of 5 by from Good sensitivityThese antibodies are very sensitive with very little background. I am delighted with this product.
Date published: 2017-06-02
Rated 5 out of 5 by from Excellent SensitivityI have tried many other suppliers which resulted in very low intensity bands, the Santa Cruz antibodies result in bright intense bands.
Date published: 2017-05-02
Rated 4 out of 5 by from Positive result in WB using purified bovinePositive result in WB using purified bovine serum albumin. -SCBT QC
Date published: 2014-01-26
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BSA Antibody (2A3E6) is rated 4.5 out of 5 by 4.
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