Brm Antibody (E-6): sc-166579
- Brm Antibody (E-6) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1531-1586 mapping at the C-terminus of Brm of human origin
- Brm Antibody (E-6) is recommended for detection of Brm of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Brm Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-166579 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Brm (E-6): sc-166579.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Brm Antibody (E-6) for WB and IHC(P) applications. These reagents are now offered in bundles with Brm Antibody (E-6) (see ordering information below).
QUICK LINKS
SEE ALSO...
Brm Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Brm protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Brm (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Brahma protein (Brm) serves as an ATPase subunit of the Drosophila melanogaster Brm complex, which is closely related to the mammalian SWI/SNF chromatin-remodeling complex. Brm plays a crucial role as a transcriptional activator of Hox genes, interacting with nearly all transcriptionally active chromatin in a manner that does not overlap with Polycomb, a known repressor of Hox gene transcription. This unique interaction is vital for the regulation of gene expression during development, as the Brm complex acts as an essential coactivator for the trithorax group protein Zeste, which binds to DNA and activates homeotic genes. Notably, a reduction in Brm function significantly decreases the association of RNA polymerase II with Drosophila salivary gland chromosomes, indicating that chromatin remodeling activity of the Brm complex is fundamental in facilitating transcription by RNA polymerase II. Furthermore, Brm acts as a dominant suppressor of the rough eye phenotype caused by a hypomorphic mutation of Drosophila cyclin E, inhibiting S phase entry by functioning downstream of cyclin E protein accumulation. The interaction of the Brm complex with chromatin may also be influenced by BAP111, which is closely associated with the Brm complex in Drosophila embryos through an HMG domain. Brm is highly expressed in unfertilized eggs and early embryos, underscoring its importance in early developmental processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Brm Antibody (E-6) References:
- The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste. | Kal, AJ., et al. 2000. Genes Dev. 14: 1058-71. PMID: 10809665
- The HMG-domain protein BAP111 is important for the function of the BRM chromatin-remodeling complex in vivo. | Papoulas, O., et al. 2001. Proc Natl Acad Sci U S A. 98: 5728-33. PMID: 11331758
- Drosophila cyclin E interacts with components of the Brahma complex. | Brumby, AM., et al. 2002. EMBO J. 21: 3377-89. PMID: 12093739
- The Drosophila BRM complex facilitates global transcription by RNA polymerase II. | Armstrong, JA., et al. 2002. EMBO J. 21: 5245-54. PMID: 12356740
- The Drosophila SNR1 (SNF5/INI1) subunit directs essential developmental functions of the Brahma chromatin remodeling complex. | Marenda, DR., et al. 2003. Mol Cell Biol. 23: 289-305. PMID: 12482982
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Brm Antibody (E-6) | sc-166579 | 200 µg/ml | $316.00 | |||
Brm Antibody (E-6): m-IgG Fc BP-HRP Bundle | sc-528997 | 200 µg Ab; 10 µg BP | $354.00 | |||
Brm Antibody (E-6): m-IgGκ BP-HRP Bundle | sc-521664 | 200 µg Ab, 40 µg BP | $354.00 | |||
Brm Antibody (E-6): m-IgG2a BP-HRP Bundle | sc-547230 | 200 µg Ab; 10 µg BP | $354.00 | |||
Brm Antibody (E-6) X | sc-166579 X | 200 µg/0.1 ml | $316.00 | |||
Brm Antibody (E-6) AC | sc-166579 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Brm Antibody (E-6) HRP | sc-166579 HRP | 200 µg/ml | $316.00 | |||
Brm Antibody (E-6) FITC | sc-166579 FITC | 200 µg/ml | $330.00 | |||
Brm Antibody (E-6) PE | sc-166579 PE | 200 µg/ml | $343.00 | |||
Brm Antibody (E-6) Alexa Fluor® 488 | sc-166579 AF488 | 200 µg/ml | $357.00 | |||
Brm Antibody (E-6) Alexa Fluor® 546 | sc-166579 AF546 | 200 µg/ml | $357.00 | |||
Brm Antibody (E-6) Alexa Fluor® 594 | sc-166579 AF594 | 200 µg/ml | $357.00 | |||
Brm Antibody (E-6) Alexa Fluor® 647 | sc-166579 AF647 | 200 µg/ml | $357.00 | |||
Brm Antibody (E-6) Alexa Fluor® 680 | sc-166579 AF680 | 200 µg/ml | $357.00 | |||
Brm Antibody (E-6) Alexa Fluor® 790 | sc-166579 AF790 | 200 µg/ml | $357.00 |