Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BRDG1 CRISPR/Cas9 KO Plasmid (m): sc-425307
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BRDG1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BRDG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BRDG1 Antibody (F-12): sc-398691
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRDG1 CRISPR/Cas9 KO Plasmid (m) | sc-425307 | 20 µg | $397.00 |
Overview
STAP1 (BRDG1) is an adaptor protein expressed in hematopoietic and immune cell lineages that coordinates intracellular signaling downstream of surface receptors. In mouse models, BRDG1 has been linked to B cell receptor-associated pathways and tyrosine kinase–dependent signaling, influencing phosphorylation cascades and transcriptional outputs that shape immune activation and differentiation. Through interactions mediated by modular binding domains, BRDG1 can affect signal integration, protein complex assembly, and downstream gene expression programs. Dysregulation of adaptor-driven signaling networks is relevant to inflammatory phenotypes and hematologic disease mechanisms, making Stap1 a useful target for studying immune signaling robustness and pathway rewiring.
BRDG1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Stap1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Stap1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Stap1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BRDG1 protein expression.
This CRISPR knockout system enables efficient generation of Stap1-deficient cell models for investigation of BRDG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Stap1 exon(s) critical for BRDG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Stap1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BRDG1 CRISPR/Cas9 KO Plasmid (m) and BRDG1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Stap1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BRDG1 HDR Plasmid (m) and BRDG1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Stap1 homology arms to support homology-directed repair at defined Stap1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.