BRDG1 Antibody (F-12): sc-398691
- BRDG1 Antibody (F-12) is a mouse monoclonal IgG1 κ BRDG1 antibody provided at 200 µg/ml
- raised against amino acids 116-295 mapping at the C-terminus of BRDG1 of human origin
- BRDG1 Antibody (F-12) is recommended for detection of BRDG1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-BRDG1 Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for BRDG1 Antibody (F-12) for WB applications. These reagents are now offered in bundles with BRDG1 Antibody (F-12) (see ordering information below).
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SEE ALSO...
BRDG1 Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects BRDG1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BRDG1 (F-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BCR downstream signaling-1 (BRDG1) protein plays a crucial role in enhancing Tec kinases activity and potentiating cellular responses downstream of B cell antigen receptor, cytokine receptor, integrin, and receptor tyrosine kinase activation, making BRDG1 vital for effective immune responses. Located primarily in the cytoplasm, BRDG1 acts as a docking protein containing a proline-rich pleckstrin homology domain and multiple candidate tyrosine phosphorylation sites, allowing BRDG1 to associate with SH2 domain-containing effector proteins. This interaction is essential for regulating signaling pathways that influence cell proliferation and differentiation. Notably, BRDG1 is preferentially phosphorylated by Tec family nonreceptor protein tyrosine kinases, such as Tec and Pyk2, which are integral to various signaling cascades. The mouse ortholog, stem cell adaptor protein 1 (STAP-1), shares 83% identity with BRDG1, highlighting the evolutionary conservation of function. Human BRDG1 protein is encoded by a gene located on chromosome 4q13.1 and consists of 295 amino acids, with transcripts being particularly abundant in the Ramos human B cell line, underscoring BRDG1′s significance in B cell biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BRDG1 Antibody (F-12) References:
- Genetic analysis of B cell antigen receptor signaling. | Kurosaki, T. 1999. Annu Rev Immunol. 17: 555-92. PMID: 10358768
- Molecular cloning of a docking protein, BRDG1, that acts downstream of the Tec tyrosine kinase. | Ohya, K., et al. 1999. Proc Natl Acad Sci U S A. 96: 11976-81. PMID: 10518561
- Molecular cloning of murine STAP-1, the stem-cell-specific adaptor protein containing PH and SH2 domains. | Masuhara, M., et al. 2000. Biochem Biophys Res Commun. 268: 697-703. PMID: 10679268
- Isoform-dependent interaction of BRDG1 with Tec kinase. | Yokohari, K., et al. 2001. Biochem Biophys Res Commun. 289: 414-20. PMID: 11716489
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRDG1 Antibody (F-12) | sc-398691 | 200 µg/ml | $322.00 | |||
BRDG1 Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-527797 | 200 µg Ab; 10 µg BP | $361.00 | |||
BRDG1 Antibody (F-12): m-IgG1 BP-HRP Bundle | sc-533170 | 200 µg Ab; 20 µg BP | $361.00 | |||
BRDG1 Antibody (F-12) AC | sc-398691 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BRDG1 Antibody (F-12) HRP | sc-398691 HRP | 200 µg/ml | $322.00 | |||
BRDG1 Antibody (F-12) FITC | sc-398691 FITC | 200 µg/ml | $336.00 | |||
BRDG1 Antibody (F-12) PE | sc-398691 PE | 200 µg/ml | $349.00 | |||
BRDG1 Antibody (F-12) Alexa Fluor® 488 | sc-398691 AF488 | 200 µg/ml | $364.00 | |||
BRDG1 Antibody (F-12) Alexa Fluor® 546 | sc-398691 AF546 | 200 µg/ml | $364.00 | |||
BRDG1 Antibody (F-12) Alexa Fluor® 594 | sc-398691 AF594 | 200 µg/ml | $364.00 | |||
BRDG1 Antibody (F-12) Alexa Fluor® 647 | sc-398691 AF647 | 200 µg/ml | $364.00 | |||
BRDG1 Antibody (F-12) Alexa Fluor® 680 | sc-398691 AF680 | 200 µg/ml | $364.00 | |||
BRDG1 Antibody (F-12) Alexa Fluor® 790 | sc-398691 AF790 | 200 µg/ml | $364.00 |