BMP-9 Antibody (H-3) recommended for detection of precursor and mature BMP-9 of mouse, rat and human origin by WB, IP, IF and ELISA

BMP-9 Antibody (H-3): sc-514211

BMP-9 Antibody (H-3) is rated 5.0 out of 5 by 1.
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Datasheets

    • Anti-BMP-9 Antibody (H-3) is a mouse monoclonal IgG1 (kappa light chain) BMP-9 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 406-425 at the C-terminus of BMP-9 of human origin
    • Anti-BMP-9 Antibody (H-3) is recommended for detection of precursor and mature BMP-9 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-BMP-9 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-514211 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BMP-9 (H-3): sc-514211.

What application is the blocking peptide sc-514211 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24

Can SC-514211 be used for P-IHC? If so, at what concentration?

Asked by: Nalini
sc-514211 is not currently recommended for IHC on formalin-fixed paraffin-embedded tissue sections, or IHC(P) because this application has not yet been tested. It is recommended for IF, or immunofluorescence or immunohistochemistry on fresh or frozen tissues or cells fixed in acetone or methanol. We recommend a starting dilution of 1:50 for IF, or a range of 1:50-1:500.
Answered by: Technical Support
Date published: 2016-09-13
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Rated 5 out of 5 by from Produced positive Western blot data of BMP Produced positive Western blot data of BMP-9 expression in non-transfected and human BMP-9 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-10-18
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