Anti-BMP-9 Antibody (H-3) is a mouse monoclonal IgG1 (kappa light chain) BMP-9 antibody provided at 200 µg/ml
specific for an epitope mapping between amino acids 406-425 at the C-terminus of BMP-9 of human origin
Anti-BMP-9 Antibody (H-3) is recommended for detection of precursor and mature BMP-9 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-BMP-9 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
blocking peptide, sc-514211 P
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BMP-9 (H-3): sc-514211.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
BMP-9 Antibody (H-3) is a high quality monoclonal BMP-9 antibody (also designated BMP-9 antibody) suitable for the detection of the BMP-9 protein of mouse, rat and human origin. BMP-9 Antibody (H-3) is available as both the non-conjugated anti-BMP-9 antibody form, as well as multiple conjugated forms of anti-BMP-9 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Bone morphogenic protein-9 (BMP-9), like other members of the TGF beta growth factor superfamily, plays an important role in tissue morphogenesis, particularly in bone and connective tissue. Additionally, liver cells such as HepG2 express receptors to BMP-9, through which it stimulates cell proliferation and regulates blood glucose concentration, an effect not observed in treatment with TGF-beta. These in vivo activities appear to be exploitable in novel therapies; research shows that addition of BMP-9 or BMP-9 encoding adenoviral vectors promote bone formation ex vivo and in immune deficient animals.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What application is the blocking peptide sc-514211 P appropriate for?
Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Answered by: Technical Support
Date published: 2017-02-24
Can SC-514211 be used for P-IHC? If so, at what concentration?
Asked by: Nalini
sc-514211 is not currently recommended for IHC on formalin-fixed paraffin-embedded tissue sections, or IHC(P) because this application has not yet been tested. It is recommended for IF, or immunofluorescence or immunohistochemistry on fresh or frozen tissues or cells fixed in acetone or methanol. We recommend a starting dilution of 1:50 for IF, or a range of 1:50-1:500.
Rated 5 out of
Produced positive Western blot data of BMPProduced positive Western blot data of BMP-9 expression in non-transfected and human BMP-9 transfected 293T whole cell lysates. -SCBT QC
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