Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BMP-7 CRISPR/Cas9 KO Plasmid (m): sc-419346
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMP-7 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BMP-7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BMP-7 Antibody (4E7): sc-53917
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMP-7 CRISPR/Cas9 KO Plasmid (m) | sc-419346 | 20 µg | $397.00 |
Overview
Bmp7 encodes bone morphogenetic protein 7 (BMP-7), a secreted TGF-β superfamily ligand that signals through type I/II BMP receptors to activate SMAD1/5/9 and coordinate transcriptional programs controlling morphogenesis and tissue homeostasis. In mouse development, BMP-7 contributes to organogenesis and patterning, with prominent roles in kidney, eye, and skeletal formation, and it also modulates epithelial–mesenchymal interactions and extracellular matrix remodeling. BMP-7-dependent signaling intersects with MAPK and other context-dependent pathways to regulate cell fate decisions, proliferation, and differentiation. Dysregulated BMP-7 activity is linked to aberrant developmental phenotypes and altered fibrotic and inflammatory responses, making Bmp7 a common target for mechanistic studies in disease-relevant models.
BMP-7 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Bmp7 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Bmp7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Bmp7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BMP-7 protein expression.
This CRISPR knockout system enables efficient generation of Bmp7-deficient cell models for investigation of BMP-7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Bmp7 exon(s) critical for BMP-7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Bmp7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BMP-7 CRISPR/Cas9 KO Plasmid (m) and BMP-7 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Bmp7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BMP-7 HDR Plasmid (m) and BMP-7 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Bmp7 homology arms to support homology-directed repair at defined Bmp7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.